Detailed Information on Publication Record
2005
Identification of interspecific barriers in the genus Lotus.
JUNGMANNOVÁ, Barbara and Jana ŘEPKOVÁBasic information
Original name
Identification of interspecific barriers in the genus Lotus.
Name in Czech
Identifikace mezidruhových bariér křižitelnosti u rodu Lotus.
Authors
JUNGMANNOVÁ, Barbara (203 Czech Republic) and Jana ŘEPKOVÁ (203 Czech Republic, guarantor)
Edition
Polsko, Acta Biologica Cracoviensia, series Botanica, 47 suppl. 1, p. 59-59, 2005
Publisher
Polish Academy of Sciences
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
40100 4.1 Agriculture, Forestry, and Fisheries
Country of publisher
Poland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.368
RIV identification code
RIV/00216224:14330/05:00012696
Organization unit
Faculty of Informatics
ISSN
UT WoS
000205501800055
Keywords in English
Lotus; interspecific barriers; clearing treatment
Změněno: 17/7/2008 08:10, prof. RNDr. Jana Řepková, CSc.
V originále
The genus Lotus is a large group comprising approximately 200 species. Lotus corniculatus is a dehiscent species with an indeterminated growth habit. It is a valuable forage species exhibiting several advantageous characteristics, such as high nutritive value, wide adaptation to soil, pH, and moisture, and low bloat hazard. However, its use has been somewhat restricted because of high seed costs. Pod shattering has been a major problem as seed loss is high due to the continuous flowering and time of pod maturity. Shattering resistance is a character of high heritability and in Lotus is considered to be controlled by more than one gene. Classical breeding approaches to reduce shattering through recurrent selection were unsuccessful (Grant 1996). Some species of Lotus reported to be indehiscent, L. conimbricensis, L. uliginosus and L. ornithopodioides, could be potential sources of genes conferred pod indehiscence. Natural interspecific hybridization does not appear in the genus Lotus. There are pre- and post-fertilization barriers of crossability. One of the pre-fertilization barriers is the incapability of pollen to germinate on the foreign stigma and the incapability of pollen tubes to grow through the style. Post-fertilization barriers cover defects in the endosperm and embryo development. Interspecific hybridization has been carried out successfully in this genus using embryo culture procedures (ODonoughue and Grant 1988; Sharma et al. 1996). The aim of our research study is determination of barriers of crossability after interspecific hybridization. An interspecific hybridization programme was undertaken involving the nonshattering species Lotus conimbricensis (2n = 12), L. uliginosus (2n = 12, 14), L. ornithopodioides (2n = 14), and they were crossed with Lotus corniculatus (2n = 24). To study the pre-fertilization barriers, callose in growing pollen tubes were stained by aniline blue. To investigate the role of post-fertilization barriers, hybrid embryo viability was traced by a clearing treatment of immature seed using chloral hydrate (Mayer et al., 1993) after modification.
In Czech
Rod Lotus obsahuje přibližně 200 druhů. L. corniculatus, hodnotná pícnina, se vyznačuje pukavými lusky, což je problematické z hlediska semenářství. Klasické metody odstraranění tohoto nedostatku jsou neúspěšné; určité možnosti nabízí mezidruhová hybridizace. Některé druhy v rámci rodu jsou nepukavé, L. conimbricensis, L. uliginosus a L. ornithopodioides, a jsou tedy vhodnými zdroji genů determinujících tento znak. Znak je determinován pravděpodobně více než jedním genem o vysoké heritabilitě. Cílem této práce bylo studium postzygotických bariér křižitelnosti a životaschopnost embryí prostřednictvím projasňování chloralhydrátem.
Links
| GD204/05/H505, research and development project |
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| MSM0021622415, plan (intention) |
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| 1G46034, research and development project |
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