KYJOVSKÁ, Zdeňka, Markéta DUDOVÁ, Tereza KOLOUCHOVÁ, Ondřej ŠTĚPÁNEK, Pavel LÍZAL, Jana ŘEPKOVÁ and Jiřina RELICHOVÁ. Genetic mapping and DNA markers for gene identification in Arabidopsis thaliana. In Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005. České Budějovice: Institute of Plant Molecular Biology ASCR, 2005, p. 47. ISBN 80-86778-16-9.
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Basic information
Original name Genetic mapping and DNA markers for gene identification in Arabidopsis thaliana
Name in Czech Identifikace genů Arabidopsis thaliana prostřednictvím genetického mapování a DNA markerů
Authors KYJOVSKÁ, Zdeňka (203 Czech Republic, guarantor), Markéta DUDOVÁ (203 Czech Republic), Tereza KOLOUCHOVÁ (203 Czech Republic), Ondřej ŠTĚPÁNEK (203 Czech Republic), Pavel LÍZAL (203 Czech Republic), Jana ŘEPKOVÁ (203 Czech Republic) and Jiřina RELICHOVÁ (203 Czech Republic).
Edition České Budějovice, Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005, p. 47-47, 2005.
Publisher Institute of Plant Molecular Biology ASCR
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/05:00012700
Organization unit Faculty of Informatics
ISBN 80-86778-16-9
Keywords in English Arabidopsis thaliana; CAPS; DNA markers; genetic mapping; microsatellites
Tags Arabidopsis thaliana, CAPS, DNA markers, genetic mapping, microsatellites
Changed by Changed by: RNDr. JUDr. Vladimír Šmíd, CSc., učo 1084. Changed: 15/1/2007 10:34.
Abstract
During the last fifteen years the efforts of geneticists and molecular biologists have been concentrated on the study and understanding of the molecular basis of plant development and identification and characterisation of new genes. In this field Arabidopsis thaliana (L.) Heynh. has played an important role as the major plant model system. It is the first plant species whose all genome was sequenced (AGI, 2000) but our knowledge of every gene function is low so far. Mutagenesis and molecular-genetic strategies have been developed to facilitate the isolation of new genes and became an attractive method for functional analysis (MEINKE et al., 2003). The main goal of our study was gene identification and localization on the genetic map of A. thaliana. We have been working with both classical (ethyl methanesulfonate, EMS) and insertional (T-DNA) collections of mutants. Altogether 10 genes were assigned on genetic map by recombination analysis of F2 plants. The known location of genes on genetic map will facilitate the decision of eventual allelism with mutants already isolated by previous authors.
Abstract (in Czech)
Hlavním cílem práce byla identifikace genů Arabidopsis thaliana a jejich lokalizace v genetické mapě. K tomu byly využity mutantní linie získané jak klasickým (EMS) tak inzerčním mutagenem (T-DNA). Celkem bylo analyzováno a zmapování 10 genů.
Links
GD204/05/H505, research and development projectName: Vývojová genetika rostlin
Investor: Czech Science Foundation, Developmental and Evolutionary Genetics of Plants
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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