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@inproceedings{583781,
author = {Vrábel, Patrik and Táborský, Petr and Ryvolová, Markéta and Řehulková, Helena and Havel, Josef and Preisler, Jan},
address = {Praha},
booktitle = {Collection Symposium Series, Biollogicaly Active Peptides},
edition = {8},
keywords = {Derivatization; Fluorescent labelling; Capillary Electrophoresis; MALDI Mass Spectrometry; Peptides and Proteins},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Praha},
isbn = {80-86241-26-2},
pages = {103-106},
publisher = {Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic},
title = {Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection},
url = {http://www.uochb.cas.cz/Symposia/BAPIX/},
year = {2005}
}
TY - JOUR
ID - 583781
AU - Vrábel, Patrik - Táborský, Petr - Ryvolová, Markéta - Řehulková, Helena - Havel, Josef - Preisler, Jan
PY - 2005
TI - Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection
PB - Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic
CY - Praha
SN - 8086241262
KW - Derivatization
KW - Fluorescent labelling
KW - Capillary Electrophoresis
KW - MALDI Mass Spectrometry
KW - Peptides and Proteins
UR - http://www.uochb.cas.cz/Symposia/BAPIX/
N2 - In this work, we apply CE-LIF, CE-UV and CE - Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOFMS) to investigate derivatization of model peptides and small proteins with rhodamine-based fluorescent reagents, which are compatible with the wavelength of frequency doubled diode pumped Nd:YAG excitation laser (532 nm). While the LIF detection mode is needed for sensitive detection, the MALDI TOFMS analysis can reveal the mass of analytes. Both derivatized and non-derivatized peptides and proteins, the number of fluorescent labels per peptide/proteins and other product of derivatization process can be detected directly from reaction mixtures or after separation. Thus, MALDI TOFMS serves for additional identification of peaks present in CELIF or CEUV electropherograms. For off-line coupling of CE with MALDI MS, subatmospheric deposition interface was used. An examples of final sensitive CELIF separation of derivatized peptides and small proteins are given. Main advantages and disadvantages of LIF, absorbance and MS detector for capillary electrophoresis of polypeptides are disscused. Attention is paid to detection limits in practical analysis.
ER -
VRÁBEL, Patrik, Petr TÁBORSKÝ, Markéta RYVOLOVÁ, Helena ŘEHULKOVÁ, Josef HAVEL and Jan PREISLER. Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection. In \textit{Collection Symposium Series, Biollogicaly Active Peptides}. 8th ed. Praha: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p.~103-106. ISBN~80-86241-26-2.
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