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@article{586528,
author = {Dostálek, Miroslav and Pistovčáková, Jana and Juřica, Jan and Tomandl, Josef and Linhart, Igor and Šulcová, Alexandra and Hadašová, Eva},
article_location = {Amsterdam, The Netherlands,},
article_number = {3},
keywords = {St John's wort; cytochrome P450; perfused rat liver; drug interaction},
language = {eng},
issn = {0024-3205},
journal = {Life Sciences},
title = {Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver},
volume = {78},
year = {2005}
}
TY - JOUR
ID - 586528
AU - Dostálek, Miroslav - Pistovčáková, Jana - Juřica, Jan - Tomandl, Josef - Linhart, Igor - Šulcová, Alexandra - Hadašová, Eva
PY - 2005
TI - Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver
JF - Life Sciences
VL - 78
IS - 3
SP - 239-244
EP - 239-244
PB - Elsevier
SN - 00243205
KW - St John's wort
KW - cytochrome P450
KW - perfused rat liver
KW - drug interaction
N2 - St. John's wort (Hypericum perforatum) is a popular over-the-counter dietary supplement and a herbal antidepressant that has been implicated in drug interactions with substrates of several cytochrome P-450 (CYP) isozymes. The effects of the St. John's wort extract (100 mg/kg, i.p., once daily for 10 days) on metabolic activity of CYP450 were assessed in the system of isolated perfused rat liver. The substrates used in this study were tolbutamide (CYP2C6), dextromethorphan (CYP2D2) and midazolam (CYP3A2). Validated HPLC method was used to quantify all compounds of interest. St. John's wort administration affected CYP activity, causing a significant decline in AUC of dextromethorphan [F(4,31) = 1511, p < 0.001; PLSD, p < 0.001] and AUC of midazolam [F(3,25) = 221, p < 0.001; PLSD, p = 0.035] and a significant increase in AUC of tolbutamide [F(3,26) = 200, p < 0.001; PLSD, p < 0.001]. St. John's wort administration resulted in a significant induction of CYP2D2 and CYP3A2, and in a significant inhibition of CYP2C6 metabolic activities.
ER -
DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Jan JUŘICA, Josef TOMANDL, Igor LINHART, Alexandra ŠULCOVÁ and Eva HADAŠOVÁ. Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver. \textit{Life Sciences}. Amsterdam, The Netherlands,: Elsevier, 2005, vol.~78, No~3, p.~239-244, 5 pp. ISSN~0024-3205.
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