Activation of different defence-related genes expression by
ergosterol

D 2005

Activation of different defence-related genes expression by ergosterol

LOCHMAN, Jan a Vladimír MIKEŠ

Základní údaje

Originální název

Activation of different defence-related genes expression by ergosterol

Název česky

Aktivace exprese obranných genů ergosterolem

Autoři

LOCHMAN, Jan (203 Česká republika) a Vladimír MIKEŠ (203 Česká republika, garant)

Vydání

Budapest, FEBS Journal, Abstract of 30th FEBS Congress, s. 470-470, 2005

Nakladatel

Blackwell publishing

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

40106 Agronomy, plant breeding and plant protection;

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/05:00014115

Organizační jednotka

Přírodovědecká fakulta

ISBN

nebylo pridelen

UT WoS

000234826102422

Klíčová slova anglicky

Elicitor; ergosterol; plant defense

Štítky

elicitor, ergosterol, plant defense

Změněno: 8. 11. 2005 09:57, prof. RNDr. Vladimír Mikeš, CSc.

Anotace

ORIG CZ

V originále

Ergosterol is the main sterol of most fungi. Production of reactive oxygen species by NADPH oxidase, alkalinization of extracellular space and increase of cytosolic concentration of calcium after the ergosterol treatment on tobacco cells was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells, even in nano-molar concentrations. Ergosterol activates a signal pathway including phospholipase A2 and protein kinase C. Here, we report about the expression of some defence-related genes after the treatment of tobacco cells and plants with nano-molar concentration of ergosterol. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,. In addition, the concentrations of some phenolic compounds and phytoalexins increased in time. On the other hand, ergosterol did not provoke any leaf necrosis or cell death and the accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea. These data shows that ergosterol is able to activate expression of a number of defence genes and could increase the resistance of plants against the pathogens.

Česky

Aktivace exprese obranných genů ergosterolem

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

LOCHMAN, Jan a Vladimír MIKEŠ. Activation of different defence-related genes expression by ergosterol. In FEBS Journal, Abstract of 30th FEBS Congress. Budapest: Blackwell publishing, 2005, s. 470. ISBN nebylo pridelen.

@inproceedings{586702,
   author = {Lochman, Jan and Mikeš, Vladimír},
   address = {Budapest},
   booktitle = {FEBS Journal, Abstract of 30th FEBS Congress},
   keywords = {Elicitor; ergosterol; plant defense},
   language = {eng},
   location = {Budapest},
   isbn = {nebylo pridelen},
   pages = {470-470},
   publisher = {Blackwell publishing},
   title = {Activation of different defence-related genes expression by ergosterol},
   year = {2005}
}

TY  - JOUR
ID  - 586702
AU  - Lochman, Jan - Mikeš, Vladimír
PY  - 2005
TI  - Activation of different defence-related genes expression by ergosterol
PB  - Blackwell publishing
CY  - Budapest
SN  - nebylo pridelen
KW  - Elicitor
KW  - ergosterol
KW  - plant defense
N2  - Ergosterol is the main sterol of most fungi. Production of reactive oxygen species by NADPH oxidase, alkalinization of extracellular space and increase of cytosolic concentration of calcium after the ergosterol treatment on tobacco cells was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells, even in nano-molar concentrations. Ergosterol activates a signal pathway including phospholipase A2 and protein kinase C. Here, we report about the expression of some defence-related genes after the treatment of tobacco cells and plants with nano-molar concentration of ergosterol. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,. In addition, the concentrations of some phenolic compounds and phytoalexins increased in time. On the other hand, ergosterol did not provoke any leaf necrosis or cell death and the accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea. These data shows that ergosterol is able to activate expression of a number of defence genes and could increase the resistance of plants against the pathogens.
ER  -

LOCHMAN, Jan a Vladimír MIKEŠ. Activation of different defence-related genes expression by ergosterol. In \textit{FEBS Journal, Abstract of 30th FEBS Congress}. Budapest: Blackwell publishing, 2005, s.~470. ISBN~nebylo~pridelen.

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