Sensitive detection of organophosphates in river water by means
of a piezoelectric biosensor

J 2005

Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor

HALÁMEK, Jan, Jan PŘIBYL, Alexander MAKOWER, Petr SKLÁDAL, Frieder SCHELLER et. al.

Basic information

Original name

Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor

Name in Czech

Citlivé stanovení organofosfátů ve vodách prostřednictvím piezoelektrického biosensoru

Authors

HALÁMEK, Jan (203 Czech Republic), Jan PŘIBYL (203 Czech Republic), Alexander MAKOWER (276 Germany), Petr SKLÁDAL (203 Czech Republic, guarantor) and Frieder SCHELLER (276 Germany)

Edition

Analytical and Bioanalytical Chemistry, Heidelberg, Springer, 2005, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.695

RIV identification code

RIV/00216224:14310/05:00014127

Organization unit

Faculty of Science

UT WoS

000231327100025

Keywords in English

Piezoelectric sensor; Quartz crystal microbalance; Acetylcholinesterase; Inhibitor

Abstract

ORIG CZ

V originále

A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors.The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane(BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) selfassembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followed anti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. An assay for detection of organophosphates (OP) was optimized. Regeneration of the sensor surface was achieved with 1 mol/L formic acid, which enabled 40 measurements with one sensor. All assays were carried out in a flowthrough arrangement. The total measurement time (binding+regeneration) was 25 min and the detection limit for different OP (paraoxon, diisopropylfluorophoshate, chlorpyriphos, and chlorfenvinphos) was down to 10^-10 mol/L (0.02 ug/L). This sensor was used for determination of organophosphate (diisopropylfluorophosphate) levels in river water samples.

In Czech

Byl vyvinut piezoelektrický biosensor pro detekci organofosfátů pomocí monovrstvy MUA-BZE-DADOO vážící cholinesterasu. Ke snížení vazby dojde v přítomnosti inhibitorů. Stanovení bylo testováno pro paraoxon, chlorpyrifos, chlorfevinfos a DFP.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

HALÁMEK, Jan, Jan PŘIBYL, Alexander MAKOWER, Petr SKLÁDAL and Frieder SCHELLER. Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor. Analytical and Bioanalytical Chemistry. Heidelberg: Springer, 2005, vol. 382, No 5, p. 1904-1911, 7 pp. ISSN 1618-2642.

@article{587323,
   author = {Halámek, Jan and Přibyl, Jan and Makower, Alexander and Skládal, Petr and Scheller, Frieder},
   article_location = {Heidelberg},
   article_number = {5},
   keywords = {Piezoelectric sensor; Quartz crystal microbalance; Acetylcholinesterase; Inhibitor},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical Chemistry},
   title = {Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor},
   url = {http://dx.doi.org/10.1007/s00216-005-3260-y},
   volume = {382},
   year = {2005}
}

TY  - JOUR
ID  - 587323
AU  - Halámek, Jan - Přibyl, Jan - Makower, Alexander - Skládal, Petr - Scheller, Frieder
PY  - 2005
TI  - Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor
JF  - Analytical and Bioanalytical Chemistry
VL  - 382
IS  - 5
SP  - 1904-1911
EP  - 1904-1911
PB  - Springer
SN  - 16182642
KW  - Piezoelectric sensor
KW  - Quartz crystal microbalance
KW  - Acetylcholinesterase
KW  - Inhibitor
UR  - http://dx.doi.org/10.1007/s00216-005-3260-y
N2  - A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors.The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane(BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) selfassembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followed anti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. An assay for detection of organophosphates (OP) was optimized. Regeneration of the sensor surface was achieved with 1 mol/L formic acid, which enabled 40 measurements with one sensor. All assays were carried out in a flowthrough arrangement. The total measurement time (binding+regeneration) was 25 min and the detection limit for different OP (paraoxon, diisopropylfluorophoshate, chlorpyriphos, and chlorfenvinphos) was down to 10^-10 mol/L (0.02 ug/L). This sensor was used for determination of organophosphate (diisopropylfluorophosphate) levels in river water samples.
ER  -

HALÁMEK, Jan, Jan PŘIBYL, Alexander MAKOWER, Petr SKLÁDAL and Frieder SCHELLER. Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: Springer, 2005, vol.~382, No~5, p.~1904-1911, 7 pp. ISSN~1618-2642.

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