Chemometric exploration of an amperometric biosensor array for
fast determination of wastewater quality

J 2005

Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality

TONNING, Erik, Svetlana SAPELNIKOVA, Jacob CHRISTENSEN, Charlotte CARLSSON, Margrethe WINTHER-NIELSEN et. al.

Základní údaje

Originální název

Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality

Název česky

Chemometrické vyhodnocování amperometrického biosensorového pole pro rychlé stanovení kvality odpadních vod

Autoři

TONNING, Erik (208 Dánsko), Svetlana SAPELNIKOVA (643 Rusko), Jacob CHRISTENSEN (208 Dánsko), Charlotte CARLSSON (752 Švédsko), Margrethe WINTHER-NIELSEN (208 Dánsko), Eva DOCK (752 Švédsko), Renáta SOLNÁ (203 Česká republika), Petr SKLÁDAL (203 Česká republika, garant), Lars NORGAARD (208 Dánsko), Tautgirdas RUZGAS (440 Litva) a Jenny EMNÉUS (752 Švédsko)

Vydání

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2005, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.463

Kód RIV

RIV/00216224:14310/05:00014128

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000232818500009

Klíčová slova anglicky

Biosensor array; Principal component analysis; Phenols; Cholinesterase; Tyrosinase; Peroxidase

Štítky

Biosensor array, Cholinesterase, peroxidase, Phenols, principal component analysis, tyrosinase

Změněno: 10. 11. 2005 22:23, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

Four wastewater samples of different treatment qualities; untreated, alarm, alert and normal, from a Swedish chemi-thermo-mechanical pulp mill and pure water were investigated using an amperometric electronic tongue in a batch cell. The aim was to explore enzymatically modified screen-printed amperometric sensors for the discrimination of wastewater quality and to counteract the inherent drift. Seven out of eight platinum electrodes on the array were modified with four different enzymes; tyrosinase, horseradish peroxidase, acetyl cholinesterase and butyryl cholinesterase. At a constant potential the current intensity on each sensor was measured for 200 s, 100 s before injection and 100 s after injection of the sample. The dynamic niosensor response curves from the eight sensors were used for principal component analysis (PCA). A simple baseline and sensitivity correction equivalent to multiplicative drift correction (MDC), using steady state intensities of reference sample (catechol) recordings, was employed. A clear pattern emerged in perfect agreement with prior knowledge of the samples explaining 97% of the variation in the data by two principal components (PCs). The first PC described the treatment quality of the samples and the second PC described the difference between treated and untreated samples. Horseradish peroxidase and pure platinum sensors were found to be the determinant sensors, while the rest did not contribute much to the discrimination. The wastewater samples were characterized by the chemical oxygen demand (COD), biological oxygen demand (BOD), total organic carbon (TOC), inhibition of nitrification, inhibition of respiration and toxicity towards Vibrio fischeri using Microtox, the freshwater alga Pseudokirchneriella subcapita and the freshwater crustacean Daphnia magna.

Česky

Biosensorové pole (8 enzymových elektrod) použité pro hodnocení kvality odpadních vod v průběhu čistícího procesu. Výsledky z jednotlivých měřících kanál byly zpracovány chemicky metodou PCA, vedoucí ke snadno interpretovatelným závěrům o stavu analyzované odpadní vody.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

TONNING, Erik, Svetlana SAPELNIKOVA, Jacob CHRISTENSEN, Charlotte CARLSSON, Margrethe WINTHER-NIELSEN, Eva DOCK, Renáta SOLNÁ, Petr SKLÁDAL, Lars NORGAARD, Tautgirdas RUZGAS a Jenny EMNÉUS. Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2005, roč. 21, č. 3, s. 608-617. ISSN 0956-5663.

@article{587324,
   author = {Tonning, Erik and Sapelnikova, Svetlana and Christensen, Jacob and Carlsson, Charlotte and WintherandNielsen, Margrethe and Dock, Eva and Solná, Renáta and Skládal, Petr and Norgaard, Lars and Ruzgas, Tautgirdas and Emnéus, Jenny},
   article_location = {Oxford},
   article_number = {3},
   keywords = {Biosensor array; Principal component analysis; Phenols; Cholinesterase; Tyrosinase; Peroxidase},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality},
   volume = {21},
   year = {2005}
}

TY  - JOUR
ID  - 587324
AU  - Tonning, Erik - Sapelnikova, Svetlana - Christensen, Jacob - Carlsson, Charlotte - Winther-Nielsen, Margrethe - Dock, Eva - Solná, Renáta - Skládal, Petr - Norgaard, Lars - Ruzgas, Tautgirdas - Emnéus, Jenny
PY  - 2005
TI  - Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality
JF  - Biosensors & Bioelectronics
VL  - 21
IS  - 3
SP  - 608-617
EP  - 608-617
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - Biosensor array
KW  - Principal component analysis
KW  - Phenols
KW  - Cholinesterase
KW  - Tyrosinase
KW  - Peroxidase
N2  - Four wastewater samples of different treatment qualities; untreated, alarm, alert and normal, from a Swedish chemi-thermo-mechanical pulp mill and pure water were investigated using an amperometric electronic tongue in a batch cell. The aim was to explore enzymatically modified screen-printed amperometric sensors for the discrimination of wastewater quality and to counteract the inherent drift. Seven out of eight platinum electrodes on the array were modified with four different enzymes; tyrosinase, horseradish peroxidase, acetyl cholinesterase and butyryl cholinesterase. At a constant potential the current intensity on each sensor was measured for 200 s, 100 s before injection and 100 s after injection of the sample. The dynamic niosensor response curves from the eight sensors were used for principal component analysis (PCA). A simple baseline and sensitivity correction equivalent to multiplicative drift correction (MDC), using steady state intensities of reference sample (catechol) recordings, was employed. A clear pattern emerged in perfect agreement with prior knowledge of the samples explaining 97% of the variation in the data by two principal components (PCs). The first PC described the treatment quality of the samples and the second PC described the difference between treated and untreated samples. Horseradish peroxidase and pure platinum sensors were found to be the determinant sensors, while the rest did not contribute much to the discrimination. The wastewater samples were characterized by the chemical oxygen demand (COD), biological oxygen demand (BOD), total organic carbon (TOC), inhibition of nitrification, inhibition of respiration and toxicity towards Vibrio fischeri using Microtox, the freshwater alga Pseudokirchneriella subcapita and the freshwater crustacean Daphnia magna.
ER  -

TONNING, Erik, Svetlana SAPELNIKOVA, Jacob CHRISTENSEN, Charlotte CARLSSON, Margrethe WINTHER-NIELSEN, Eva DOCK, Renáta SOLNÁ, Petr SKLÁDAL, Lars NORGAARD, Tautgirdas RUZGAS a Jenny EMNÉUS. Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2005, roč.~21, č.~3, s.~608-617. ISSN~0956-5663.

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