New insight into mediator function of yeast Rad52 protein

D 2005

New insight into mediator function of yeast Rad52 protein

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN et. al.

Základní údaje

Originální název

New insight into mediator function of yeast Rad52 protein

Název česky

Nový pohled na mediátorovou funkci proteinu Rad52

Autoři

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN a Uffe H. MORTENSEN

Vydání

Bratislava, Slovakia, XXIIth International Conference on Yeast Genetics and Molecular Biology, 90 s. 2005

Nakladatel

ASM Conferences Program

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Recombination; DNA repair; Rad52; mediator; yeast

Štítky

DNA repair, mediator, Rad52, recombination, Yeast

Změněno: 30. 6. 2009 22:56, doc. Mgr. Lumír Krejčí, Ph.D.

Anotace

ORIG CZ

V originále

Homologous recombination (HR) represents an important means for the repair of chromosome breaks induced by exogenous agents (e.g. ionizing radiation) or that arise endogenously (e.g. replication of a damaged DNA template). The elimination of these breaks is critical for the maintenance of genome stability. DNA strand break repair by HR is mediated by genes of the RAD52 epistasis group, whose structure and function are highly conserved among eukaryotes. Rad52 protein plays central role in this process. It binds and anneals complemtary ssDNA, self-associate to form multimeric rings, interacts with Rad51, and promotes nucleation of Rad51 onto ssDNA. Here, we show that Rad52 contains second DNA binding domain that is capable of mediating Rad51-catalyzed strand exchange reaction. The molecular mechanism of Rad52 function in homologous recombination is disccused.

Česky

V této praci prezentujeme, že Rad52 protein obsahuje druhou DNA vazebnou doménu, která je schopna zprostredkovat Rad51-katalyzovanou výměnu řetězců. Diskutujeme molekulární mechanismus homologní rekombinace.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN a Uffe H. MORTENSEN. New insight into mediator function of yeast Rad52 protein. In XXIIth International Conference on Yeast Genetics and Molecular Biology. Bratislava, Slovakia: ASM Conferences Program, 2005, 90 s.

@inproceedings{588622,
   author = {Krejci, Lumir and Plate, Iben and Sung, Patrick and Sehorn, Michael and Bussen, Wendy and Mortensen, Uffe H.},
   address = {Bratislava, Slovakia},
   booktitle = {XXIIth International Conference on Yeast Genetics and Molecular Biology},
   keywords = {Recombination; DNA repair; Rad52; mediator; yeast},
   language = {eng},
   location = {Bratislava, Slovakia},
   publisher = {ASM Conferences Program},
   title = {New insight into mediator function of yeast Rad52 protein},
   url = {http://www.yeast2005.org/},
   year = {2005}
}

TY  - JOUR
ID  - 588622
AU  - Krejci, Lumir - Plate, Iben - Sung, Patrick - Sehorn, Michael - Bussen, Wendy - Mortensen, Uffe H.
PY  - 2005
TI  - New insight into mediator function of yeast Rad52 protein
PB  - ASM Conferences Program
CY  - Bratislava, Slovakia
KW  - Recombination
KW  - DNA repair
KW  - Rad52
KW  - mediator
KW  - yeast
UR  - http://www.yeast2005.org/
L2  - http://www.yeast2005.org/
N2  - Homologous recombination (HR) represents an important means for the repair of chromosome breaks induced by exogenous agents (e.g. ionizing radiation) or that arise endogenously (e.g. replication of a damaged DNA template). The elimination of these breaks is critical for the maintenance of genome stability. DNA strand break repair by HR is mediated by genes of the RAD52 epistasis group, whose structure and function are highly conserved among eukaryotes. Rad52 protein plays central role in this process. It binds and anneals complemtary ssDNA, self-associate to form multimeric rings, interacts with Rad51, and promotes nucleation of Rad51 onto ssDNA. Here, we show that Rad52 contains second DNA binding domain that is capable of mediating Rad51-catalyzed strand exchange reaction. The molecular mechanism of Rad52 function in homologous recombination is disccused.
ER  -

KREJCI, Lumir, Iben PLATE, Patrick SUNG, Michael SEHORN, Wendy BUSSEN a Uffe H. MORTENSEN. New insight into mediator function of yeast Rad52 protein. In \textit{XXIIth International Conference on Yeast Genetics and Molecular Biology}. Bratislava, Slovakia: ASM Conferences Program, 2005, 90 s.

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