Další formáty:
BibTeX
LaTeX
RIS
@article{596865, author = {Moskovets, Eugene and Preisler, Jan and Chen, H. S. and Rejtar, Tomáš and Andreev, Viktor and Karger, Barry L.}, article_location = {Washington, D.C., USA}, article_number = {3}, keywords = {MALDI; TOF; mass spectrometry; time of flight; high-throughput}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser}, volume = {78}, year = {2006} }
TY - JOUR ID - 596865 AU - Moskovets, Eugene - Preisler, Jan - Chen, H. S. - Rejtar, Tomáš - Andreev, Viktor - Karger, Barry L. PY - 2006 TI - High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser JF - Analytical Chemistry VL - 78 IS - 3 SP - 912-919 EP - 912-919 PB - American Chemical Society SN - 00032700 KW - MALDI KW - TOF KW - mass spectrometry KW - time of flight KW - high-throughput N2 - A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2 kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axial mass spectrometer, while maintaining comparable limits of detection (200 amole of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10,000 vs. 14,000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z <2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2 kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots per second (with 800 shots/spot). The overall performance of the fast MALDI-TOF MS instrument was demonstrated by the acquisition, in 12 minutes, of an LC-MS dataset from a plate of 650 fractions collected during LC separation of an 16O/18O differentially-labeled proteomic sample of a tryptic digest of an E.coli lysate. ER -
MOSKOVETS, Eugene, Jan PREISLER, H. S. CHEN, Tomáš REJTAR, Viktor ANDREEV a Barry L. KARGER. High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2006, roč.~78, č.~3, s.~912-919. ISSN~0003-2700.
|