Amperometric flow-injection determination of phenolic compounds
using a biosensor with immobilised laccase, peroxidase and
tyrosinase

J 2005

Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase

SOLNÁ, Renáta a Petr SKLÁDAL

Základní údaje

Originální název

Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase

Název česky

Amperometrický průtočný biosensor pro stanovení fenolických sloučenin s imobilisovanou lakasou, peroxidasou a tyrosinasou

Autoři

SOLNÁ, Renáta (203 Česká republika) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2005, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.189

Kód RIV

RIV/00216224:14310/05:00014346

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000234031500005

Klíčová slova anglicky

electrochemical biosensor; enzyme electrode; phenol; catechol; waste water

Štítky

catechol, electrochemical biosensor, Enzyme electrode, PHENOL, waste water

Změněno: 2. 1. 2006 13:27, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.

Česky

Byl vyvinut multikanálový elektrochemický biosensor pro stanovení fenolických látek v odpadních vodách pomocí enzymů specifických k fenolům: lakasa, peroxidasy a tyrosinasa.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

SOLNÁ, Renáta a Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2005, roč. 17, č. 23, s. 2137-2146. ISSN 1040-0397.

@article{598204,
   author = {Solná, Renáta and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {23},
   keywords = {electrochemical biosensor; enzyme electrode; phenol; catechol; waste water},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase},
   volume = {17},
   year = {2005}
}

TY  - JOUR
ID  - 598204
AU  - Solná, Renáta - Skládal, Petr
PY  - 2005
TI  - Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
JF  - Electroanalysis
VL  - 17
IS  - 23
SP  - 2137-2146
EP  - 2137-2146
PB  - Wiley-VCH Verlag GmbH
SN  - 10400397
KW  - electrochemical biosensor
KW  - enzyme electrode
KW  - phenol
KW  - catechol
KW  - waste water
N2  - A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.
ER  -

SOLNÁ, Renáta a Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2005, roč.~17, č.~23, s.~2137-2146. ISSN~1040-0397.

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