| SOLNÁ, Renáta a Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2005, roč. 17, č. 23, s. 2137-2146. ISSN 1040-0397. |
Další formáty:
BibTeX
LaTeX
RIS
@article{598204,
author = {Solná, Renáta and Skládal, Petr},
article_location = {Weinheim},
article_number = {23},
keywords = {electrochemical biosensor; enzyme electrode; phenol; catechol; waste water},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase},
volume = {17},
year = {2005}
}
TY - JOUR
ID - 598204
AU - Solná, Renáta - Skládal, Petr
PY - 2005
TI - Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
JF - Electroanalysis
VL - 17
IS - 23
SP - 2137-2146
EP - 2137-2146
PB - Wiley-VCH Verlag GmbH
SN - 10400397
KW - electrochemical biosensor
KW - enzyme electrode
KW - phenol
KW - catechol
KW - waste water
N2 - A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.
ER -
SOLNÁ, Renáta a Petr SKLÁDAL. Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2005, roč.~17, č.~23, s.~2137-2146. ISSN~1040-0397.
|
