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@article{617344, author = {Havliš, Jan and Štosová, Tatiana and Šebela, Marek and Lenobl, René}, article_location = {Praha}, article_number = {12}, keywords = {TANDEM MASS-SPECTROMETRY; AMINO-ACID SEQUENCE; PROTEIN IDENTIFICATION; POLYACRYLAMIDE-GELS; QUADRUPOLE-TIME; LYSOBACTER-ENZYMOGENES; CHEMICAL-MODIFICATION; CARBOXYPEPTIDASE-Y; PSEUDOMONAS-FRAGI; MEMBRANE-PROTEINS}, language = {cze}, issn = {0009-2770}, journal = {Chemické listy}, title = {Proteolytické enzymy: význam pro proteomiku}, volume = {99}, year = {2005} }
TY - JOUR ID - 617344 AU - Havliš, Jan - Štosová, Tatiana - Šebela, Marek - Lenobl, René PY - 2005 TI - Proteolytické enzymy: význam pro proteomiku JF - Chemické listy VL - 99 IS - 12 SP - 896-905 EP - 896-905 PB - Czech Chemical Society SN - 00092770 KW - TANDEM MASS-SPECTROMETRY KW - AMINO-ACID SEQUENCE KW - PROTEIN IDENTIFICATION KW - POLYACRYLAMIDE-GELS KW - QUADRUPOLE-TIME KW - LYSOBACTER-ENZYMOGENES KW - CHEMICAL-MODIFICATION KW - CARBOXYPEPTIDASE-Y KW - PSEUDOMONAS-FRAGI KW - MEMBRANE-PROTEINS N2 - Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are also mentioned. Finally, new perspectives of proteolysis in shotgun proteomics are outlined. ER -
HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA a René LENOBL. Proteolytické enzymy: význam pro proteomiku. \textit{Chemické listy}. Praha: Czech Chemical Society, 2005, roč.~99, č.~12, s.~896-905. ISSN~0009-2770.
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