2005
Genomic imbalance detected in glioblastoma multigorme using high-resolution comparative genomic hybridization
KUGLÍK, Petr, Vladimíra VRANOVÁ, Pavel CEJPEK, Eva NEESALOVÁ, Martina PEŠÁKOVÁ et. al.Základní údaje
Originální název
Genomic imbalance detected in glioblastoma multigorme using high-resolution comparative genomic hybridization
Název česky
Abnormality chromozomů detegované u multiformního glioblastomu pomocí techniky komparativní genomové hybridizace s vysokým rozlišením
Autoři
KUGLÍK, Petr (203 Česká republika, garant), Vladimíra VRANOVÁ (703 Slovensko), Pavel CEJPEK (203 Česká republika), Eva NEESALOVÁ (203 Česká republika), Martina PEŠÁKOVÁ (203 Česká republika) a Jiina RELICHOVÁ (203 Česká republika)
Vydání
UK, Chromosome Research, s. 87-87, 2005
Nakladatel
Springer
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/05:00014596
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Glioblastoma multiforme; chromosome aberrations; HR-CGH; FISH
Změněno: 15. 1. 2007 11:07, doc. RNDr. Petr Kuglík, CSc.
V originále
Glioblastoma multiforme (GBM) is the most common form of primary neoplasm occurring in the central nervous system of adults. The tumour can develop from a low-degree or anaplastic astrocyte secondary glioblastoma, but more frequently it manifests itself with a short clinical anamnesi as primary glioblastoma. Cytogenetic diagnostic methods form an integral part glioblastoma diagnostics, however, conventional cytogenetics has been unable to identify consistent chromosomal aberrations in this group of tumours. Thus, more advanced molecular cytogenetic approaches are required to study the relationship between chromosomal instability and patient prognosis. The aim of this study was to screen for chromosomal imbalances in GBM by CGH and modified HR-CGH. Aim was to determine chromosomal changes on primary tumours and evaluate intratumoral genetic heterogenity by comparing gains and losses of chromosomes in central and peripheral areas of tumours. In this study, chromosomal abnormalities in 22 cases of GBM were analysed using CGH. A total of 99 different changes were observed (with a median of 5 changes per case). The most prominent gains were found in chromosomes 7, 19, 3q, 12, Xp. The losses concerned mainly chromosome 10, 6, 13q, 14q, 1p. Losses of chromosomal material (56) were more frequent than gains (43). To further increase detection rate of chromosomal imbalances, we applied HR-CGH in 11 cases GBM. HR-CGH revealed more aberrations per patiens than did CGH. A total of 172 abnormalities were found with HR-CGH (with a median of 9 changes per case). Low frequent aberrant clones with whole chromosome gains as well as losses of chromosomal segments were further revealed by HR-CGH. Furthermore, our data confirmed genetic heterogenity of GBM; gains and losses of DNA sequences have been found to a much lesser extend in central areas compared to the peripheral areas of tumours.
Česky
Práce se zabývá výskytem specifických chromozomových abnormalit u mozkového nádoru multiformního glioblastomu, které byly studovány pomocí techniky komparativní genomové hybridizace s vysokým rozlišením.
Návaznosti
| MSM0021622415, záměr |
| ||
| OC B19.001, projekt VaV |
|