Evaluating the functional diversity of soil microorganisms
using the BIOLOG method in situ studies and laboratory tests of
toxicity

D 2004

Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity

HOFMAN, Jakub, Jiří ŠVIHÁLEK a Martin NEČAS

Základní údaje

Originální název

Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity

Název česky

Hodnocení funkční diverzity mikroorganismů metodou BIOLOG v reálných studiích i v testech toxicity

Autoři

HOFMAN, Jakub (203 Česká republika, garant), Jiří ŠVIHÁLEK (203 Česká republika) a Martin NEČAS (203 Česká republika)

Vydání

České Budějovice, Czech Republic, Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates, od s. 67-72, 6 s. 2004

Nakladatel

Institute of Soil Biology AS CR

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/04:00019959

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-86525-03-1

Klíčová slova anglicky

soil; microorganisms; diversity; BIOLOG

Štítky

BIOLOG, diversity, microorganisms, soil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2010 20:46, prof. RNDr. Jakub Hofman, Ph.D.

Anotace

ORIG CZ

V originále

This contribution is short example of one field study and three laboratory experiments, which were carried out to answer the question, if the BIOLOG method is suitable to be sensitive indicator of soil contamination in soil ecotoxicology. The BIOLOG main difficulty is introduced especially as effect of inoculum density which causes that information about overall catabolic activity cannot be separated from information about functional diversity, i.e. differences in substrate utilization patterns. In our case field study, twelve soils were measured from area around an industrial source. Soils were compared separately according their catabolic activity and relative diversity. In laboratory toxicity tests, cadmium for validation and toxaphene or chlorinated paraffin as problematic environmental pollutants were evaluated. Cadmium validated BIOLOG to be sensitive as overall physiological activity endpoint. However, there were no diversity changes induced. Toxaphene revealed no effect even at 10 ppm level. The highest levels (10 000 ppm) of both chlorinated paraffin and dodecane stimulated physiological activity and also induced some changes in physiological profile. To conclude, BIOLOG is promising for soil ecotoxicology studies but separated assessment of differences in physiological activity from differences in functional diversity, i.e. substrate utilization patterns, are strongly required to interpret the data correctly.

Česky

viz. ang.

Návaznosti

GA525/03/0367, projekt VaV

Název: Ekotoxikologie persistentních organických polutantů životního prostředí

Investor: Grantová agentura ČR, Ekotoxikologie persistentních organických polutantů životního prostředí

GP525/04/P159, projekt VaV

Název: Vliv nově rozpoznávaných prioritních perzistentních organických polutantů (POPs) na organismy v půdním ekosystému

Investor: Grantová agentura ČR, Vliv nově rozpoznávaných prioritních perzistentních organických polutantů (POPs) na organismy v půdním ekosystému

Citovat

HOFMAN, Jakub, Jiří ŠVIHÁLEK a Martin NEČAS. Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity. In Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates. České Budějovice, Czech Republic: Institute of Soil Biology AS CR, 2004, s. 67-72. ISBN 80-86525-03-1.

@inproceedings{619105,
   author = {Hofman, Jakub and Švihálek, Jiří and Nečas, Martin},
   address = {České Budějovice, Czech Republic},
   booktitle = {Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates},
   keywords = {soil; microorganisms; diversity; BIOLOG},
   language = {eng},
   location = {České Budějovice, Czech Republic},
   isbn = {80-86525-03-1},
   pages = {67-72},
   publisher = {Institute of Soil Biology AS CR},
   title = {Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity},
   year = {2004}
}

TY  - JOUR
ID  - 619105
AU  - Hofman, Jakub - Švihálek, Jiří - Nečas, Martin
PY  - 2004
TI  - Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity
PB  - Institute of Soil Biology AS CR
CY  - České Budějovice, Czech Republic
SN  - 8086525031
KW  - soil
KW  - microorganisms
KW  - diversity
KW  - BIOLOG
N2  - This contribution is short example of one field study and three laboratory experiments, which were carried out to answer the question, if the BIOLOG method is suitable to be sensitive indicator of soil contamination in soil ecotoxicology. The BIOLOG main difficulty is introduced especially as effect of inoculum density which causes that information about overall catabolic activity cannot be separated from information about functional diversity, i.e. differences in substrate utilization patterns. In our case field study, twelve soils were measured from area around an industrial source. Soils were compared separately according their catabolic activity and relative diversity. In laboratory toxicity tests, cadmium for validation and toxaphene or chlorinated paraffin as problematic environmental pollutants were evaluated. Cadmium validated BIOLOG to be sensitive as overall physiological activity endpoint. However, there were no diversity changes induced. Toxaphene revealed no effect even at 10 ppm level. The highest levels (10 000 ppm) of both chlorinated paraffin and dodecane stimulated physiological activity and also induced some changes in physiological profile. To conclude, BIOLOG is promising for soil ecotoxicology studies but separated assessment of differences in physiological activity from differences in functional diversity, i.e. substrate utilization patterns, are strongly required to interpret the data correctly.
ER  -

HOFMAN, Jakub, Jiří ŠVIHÁLEK a Martin NEČAS. Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity. In \textit{Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates}. České Budějovice, Czech Republic: Institute of Soil Biology AS CR, 2004, s.~67-72. ISBN~80-86525-03-1.

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