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@inproceedings{619409, author = {Pašková, Veronika and Hilscherová, Klára and Bláha, Luděk}, address = {Brno}, booktitle = {Book of abstracts of ECOTOX 2005 - Advances and Trends in Ecotoxicology}, edition = {1st ed.}, keywords = {phytotoxicity; Sinapis alba; Triticum aestivum; Phaseolus vulgaris; PAHs; N-PAHs; lipid peroxidation; glutathione; glutathione-S-transferase; glutathione reductase; glutathione peroxidase}, language = {eng}, location = {Brno}, isbn = {80-210-3799-7}, pages = {187-187}, publisher = {Masaryk University Brno}, title = {Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues}, year = {2005} }
TY - JOUR ID - 619409 AU - Pašková, Veronika - Hilscherová, Klára - Bláha, Luděk PY - 2005 TI - Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues PB - Masaryk University Brno CY - Brno SN - 8021037997 KW - phytotoxicity KW - Sinapis alba KW - Triticum aestivum KW - Phaseolus vulgaris KW - PAHs KW - N-PAHs KW - lipid peroxidation KW - glutathione KW - glutathione-S-transferase KW - glutathione reductase KW - glutathione peroxidase N2 - In framework of this diploma thesis were studied toxic effects of polyaromatic hydrocarbons (phenanthrene, anthracene, fluorene) and its N-heterocyclic derivates (phenanthridine, 1,10-phenanthroline, 4,7-phenanthroline, 1,7-phenanthroline, benzo[h]quinoline, acridine, carbazole) on terrestrial plants white mustard (Sinapis alba), wheat (Triticum aestivum) and bean (Phaseolus vulgaris). Tests of acute phytotoxicity were carried out in laboratories of the centre RECETOX, Masaryk University in Brno. The classic morphological parametres (shoot length, hypocotyl length and their weights) and germinability of seeds were observed. The parameter of lipid peroxidation (TBARS), as well as the level and activity of antioxidative components of plant metabolism (glutathione, glutathione-S-transferase, glutathione reductase, glutathione peroxidase) were measured to identify the rate of induction of oxidative stress. Evidently new informations about in the meanwhile little studied group of environmental toxicants (PAHs versus aza-PAHs) were established in this work. The results of this study implicate that homocyclic polyaromatic hydrocarbons are less toxic than their N-substituents, with respect to interference in morphological parametres. S. alba and T. aestivum were more sensitive than P. vulgaris. Chemicals with the strongest inhibition effect on the process of germination and the growth of plants were determined (phenanthridine, acridine, benzo[h]quinoline, 1,10 - and 1,7-phenanthroline). Furthermore were found out that fluorene, carbazole, acridine, phenanthrene, phenanthridine, benzo[h]quinoline and 1,7-phenanthroline significantly increased lipid peroxidation. All tested chemicals significantly induced the increase of activity of detoxification plant enzymes, in case of increased level of glutathion significantly caused all tested chemicals except 1,10- and 4,7-phenanthroline. ER -
PAŠKOVÁ, Veronika, Klára HILSCHEROVÁ a Luděk BLÁHA. Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues. In \textit{Book of abstracts of ECOTOX 2005 - Advances and Trends in Ecotoxicology}. 1st ed. Brno: Masaryk University Brno, 2005, s.~187-187, 248 s. ISBN~80-210-3799-7.
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