Quantitative exploration of T-tubular membrane parameters in a
model of rat ventricular cardiomyocyte

J 2005

Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte

PÁSEK, Michal; Jiří ŠIMURDA; Clive ORCHARD a Georges CHRISTÉ

Základní údaje

Originální název

Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte

Název česky

Kvantitativní vyšetření parametrů tubulární membrány prostednictvím modelu komorové buňky potkana

Autoři

PÁSEK, Michal (203 Česká republika, garant); Jiří ŠIMURDA (203 Česká republika); Clive ORCHARD (826 Velká Británie a Severní Irsko) a Georges CHRISTÉ (250 Francie)

Vydání

Journal of Molecular and Cellular Cardiology, Elsevier Ltd. 2005, 0022-2828

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.872

Kód RIV

RIV/00216224:14110/05:00014820

Organizační jednotka

Lékařská fakulta

UT WoS

000230435000042

Klíčová slova anglicky

cardiac cell; tubular system; quantitative modelling

Štítky

Cardiac cell, Quantitative modelling, tubular system

Změněno: 25. 6. 2009 16:08, doc. Ing. Michal Pásek, Ph.D.

Anotace

ORIG CZ

V originále

Formamide-induced detubulation of rat ventricular cardiac myocytes causes an ~32% decrease of membrane capacitance (Despa et al, 2003). Detailed microscopic analysis of the transverse-axial tubular system (TATS) by Soeller and Cannel (1999) showed a mean tubule diameter of 255 nm, a mean segment length of 6.87 ľm, a tubule density of 0.3 ľm-2, fractional volume of 3.6% and TATS membrane area to cell volume ratio of 0.44 ľm2/ľm3. These parameters can be approximately respected when the fraction of the total sarcolemma within the TATS is 56%. To explore possible causes for this discrepancy we used a quantitative model of the rat ventricular myocyte that included a description of TATS. Two explanations were found that could account quantitatively for the discrepancy: (i) incomplete detubulation leaving electrical access to a large area of TATS membrane, due to the branched TATS network. The estimate of Despa et al. could be met if 42% of TATS remained accessible after detubulation. (ii) the specific membrane capacitance of TATS membrane is lower than that of the surface sarcolemma (set to 1 ľFcm-2). Such a difference may result from the different composition of the two membranes. To account for the discrepancy, the specific capacitance of the TATS membrane must be decreased to 0.37 ľF.cm-2. A combination of both explanations may contribute to the observed discrepancy, e.g. 15% TATS membrane area remaining accessible with specific capacitance of 0.47 ľF.cm-2.

Česky

Cílem práce bylo objasnit rozdíly v publikovaných parametrech transverzálně-axiálního tubulárního systému potkana prostřednictvím matematického modelu.

Návaznosti

MSM0021622402, záměr

Název: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časná diagnostika a léčba kardiovaskulárních chorob

Citovat

PÁSEK, Michal; Jiří ŠIMURDA; Clive ORCHARD a Georges CHRISTÉ. Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte. Journal of Molecular and Cellular Cardiology. Elsevier Ltd., 2005, roč. 39, č. 1, s. 177-178. ISSN 0022-2828.

@article{622348,
   author = {Pásek, Michal and Šimurda, Jiří and Orchard, Clive and Christé, Georges},
   article_number = {1},
   keywords = {cardiac cell; tubular system; quantitative modelling},
   language = {eng},
   issn = {0022-2828},
   journal = {Journal of Molecular and Cellular Cardiology},
   title = {Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte},
   volume = {39},
   year = {2005}
}

TY  - JOUR
ID  - 622348
AU  - Pásek, Michal - Šimurda, Jiří - Orchard, Clive - Christé, Georges
PY  - 2005
TI  - Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte
JF  - Journal of Molecular and Cellular Cardiology
VL  - 39
IS  - 1
SP  - 177-178
EP  - 177-178
PB  - Elsevier Ltd.
SN  - 00222828
KW  - cardiac cell
KW  - tubular system
KW  - quantitative modelling
N2  - Formamide-induced detubulation of rat ventricular cardiac myocytes causes an ~32% decrease of membrane capacitance (Despa et al, 2003). Detailed microscopic analysis of the transverse-axial tubular system (TATS) by Soeller and Cannel (1999) showed a mean tubule diameter of 255 nm, a mean segment length of 6.87 ľm, a tubule density of 0.3 ľm-2, fractional volume of 3.6% and TATS membrane area to cell volume ratio of 0.44 ľm2/ľm3. These parameters can be approximately respected when the fraction of the total sarcolemma within the TATS is 56%. To explore possible causes for this discrepancy we used a quantitative model of the rat ventricular myocyte that included a description of TATS. Two explanations were found that could account quantitatively for the discrepancy: (i) incomplete detubulation leaving electrical access to a large area of TATS membrane, due to the branched TATS network. The estimate of Despa et al. could be met if 42% of TATS remained accessible after detubulation. (ii) the specific membrane capacitance of TATS membrane is lower than that of the surface sarcolemma (set to 1 ľFcm-2). Such a difference may result from the different composition of the two membranes. To account for the discrepancy, the specific capacitance of the TATS membrane must be decreased to 0.37 ľF.cm-2. A combination of both explanations may contribute to the observed discrepancy, e.g. 15% TATS membrane area remaining accessible with specific capacitance of 0.47 ľF.cm-2.
ER  -

PÁSEK, Michal; Jiří ŠIMURDA; Clive ORCHARD a Georges CHRISTÉ. Quantitative exploration of T-tubular membrane parameters in a model of rat ventricular cardiomyocyte. \textit{Journal of Molecular and Cellular Cardiology}. Elsevier Ltd., 2005, roč.~39, č.~1, s.~177-178. ISSN~0022-2828.

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