2005
Antiproliferative activities of selected benzo[c]phenanthridine alkaloids.
SLUNSK, Zdenka, Iva SLANINOVÁ, Jiří ŠINKORA, Marcela VLKOVÁ, Eva TBORSKÁ et. al.Základní údaje
Originální název
Antiproliferative activities of selected benzo[c]phenanthridine alkaloids.
Název česky
Antiproliferativní aktivity vybraných benzo[c]phenanthridinových alkaloidů
Autoři
SLUNSK, Zdenka (203 Česká republika), Iva SLANINOVÁ (203 Česká republika, garant), Jiří ŠINKORA (203 Česká republika), Marcela VLKOVÁ (203 Česká republika) a Eva TBORSKÁ (203 Česká republika)
Vydání
Olomouc, CR, Biomedical Papers, od s. 57-58, 2 s. 2005
Nakladatel
Palacky University, Olomouc
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/05:00013263
Organizační jednotka
Lékařská fakulta
ISBN
1213-8118
Klíčová slova anglicky
benzo[c]phenanthridine alkaloid; cytotoxicity; apoptosis; MTT assay
Příznaky
Mezinárodní význam
Změněno: 19. 1. 2007 09:58, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Quaternary benzo[c]phenanthridine alkaloids (QBA) fall into a group of isochinoline alkaloids that have phenylalanin as their precursor. They are isolated from many plant species for instance from Papaveraceae, Fumariaceae and Rutaceae families. Sanguinarin and chelerytrin are the two best known QBA and their biological activities have been widely studied (1,2,3). In addition to them about 10 other QBA differing only by substitution on the aromatic rings have been isolated as a minor components in several species. The informations about biological effects of this minor QBA are rare. In this study the biological effect of sanguinarine (SA), chelerythrine (CHE) and their derinates sanguirubine (SR), chelirubine (CHR) and macarpine (MA) were tested. One normal (skin fibroblasts) and 3 tumour cell lines (HeLa; A 431; HL-60) were used as a model object. Cytotoxicity (IC50) and apoptosis on living cells were studied. After 72 hours, MTT assay was performed to evaluate the in vitro cytotoxicity induced by tested alkaloids at concentration 0,01-5ěg/ml. Promyelocytic leukemia cell line HL-60 has been proved the most sensitive to the alkaloid treatment followed by skin fibroblasts, while the carcinoma cell lines HeLa and A-431 appeared much more resistant. Cytotoxicity of individual alkaloids descended (the value of IC50 increased) in this order CHE > MA > SA > SR > CHR. For apoptosis detection morphology of nuclei of alkaloid treated HL-60 cells after DAPI staining was studied and flow cytometric analysis using Annexin V-FITC and PI was applied. SA, CHE, MA and SR appeared to be inductors of apoptosis. This work was supported by the grants 525/040017 and 301/03/H005 from the Grant agency of Czech republic and MSM0021622415 from Ministry of Education of the Czech Republic
Česky
Kvartern benzo[c]phenanthridine alkaloids (QBA) pat do skupiny isochinolinovch alkaloid, jejich prekursorem je phenylalanin. Vyskytuj se v rostlinch eled Papaveraceae, Fumariaceae a Rutaceae. Nejznmj a nejlpe prostudovan jsou Sanguinarin and chelerytrin, informace o biologickch aktivitch minoritnch derivt jsou vzcn. Tato studie se zabv srovnnm antiproliferan aktivity sanguinarinu (SA), chelerythrinu (CHE) a minoritnch derivt sanguirubinu (SR), chelirubinu (CHR) a macarpinu (MA) na linii normlnch lidskch konch fibroblast a 3 ndorov bunn linie (HeLa; A 431; HL-60). Antiproliferan aktivita byla stanovena po 72 hodinov kultivaci pomoc MTT assae, schopnost indukovat apoptzu byla studovna pomoc kombinace Anexinu V a propidium jodidu.
Návaznosti
| GA525/04/0017, projekt VaV |
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| GD301/03/H005, projekt VaV |
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| MSM0021622415, záměr |
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