| KOLÁŘ, Milan, Roman PANTŮČEK, Iva VÁGNEROVÁ, Michaela KESSELOVÁ, Pavel SAUER, Ivana MATOUŠKOVÁ, Jiří DOŠKAŘ, Dagmar KOUKALOVÁ and Petr HEJNAR. Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic. Clinical Microbiology and Infection. Oxford, United Kingdom: Blackwell Publishing Ltd., 2006, vol. 12, No 4, p. 353-360. ISSN 1198-743X. |
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@article{629925,
author = {Kolář, Milan and Pantůček, Roman and Vágnerová, Iva and Kesselová, Michaela and Sauer, Pavel and Matoušková, Ivana and Doškař, Jiří and Koukalová, Dagmar and Hejnar, Petr},
article_location = {Oxford, United Kingdom},
article_number = {4},
keywords = {Enterococcus faecium; molecular epidemiology; pulsed-field gel electrophoresis; vancomycin-resistant enterococci},
language = {eng},
issn = {1198-743X},
journal = {Clinical Microbiology and Infection},
title = {Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic},
url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1469-0691.2006.01365.x},
volume = {12},
year = {2006}
}
TY - JOUR
ID - 629925
AU - Kolář, Milan - Pantůček, Roman - Vágnerová, Iva - Kesselová, Michaela - Sauer, Pavel - Matoušková, Ivana - Doškař, Jiří - Koukalová, Dagmar - Hejnar, Petr
PY - 2006
TI - Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic
JF - Clinical Microbiology and Infection
VL - 12
IS - 4
SP - 353-360
EP - 353-360
PB - Blackwell Publishing Ltd.
SN - 1198743X
KW - Enterococcus faecium
KW - molecular epidemiology
KW - pulsed-field gel electrophoresis
KW - vancomycin-resistant enterococci
UR - http://www.blackwell-synergy.com/doi/abs/10.1111/j.1469-0691.2006.01365.x
N2 - This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.
ER -
KOLÁŘ, Milan, Roman PANTŮČEK, Iva VÁGNEROVÁ, Michaela KESSELOVÁ, Pavel SAUER, Ivana MATOUŠKOVÁ, Jiří DOŠKAŘ, Dagmar KOUKALOVÁ and Petr HEJNAR. Genotypic characterisation of vancomycin-resistant $<$I$>$Enterococcus faecium$<$/I$>$ isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic. \textit{Clinical Microbiology and Infection}. Oxford, United Kingdom: Blackwell Publishing Ltd., 2006, vol.~12, No~4, p.~353-360. ISSN~1198-743X.
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