Binding of different monosaccharides by lectin PA-IIL from
Pseudomonas aeruginosa: Thermodynamics data correlated with
X-ray structures

J 2006

Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures

SABIN, Charles, Edward P. MITCHELL, Martina POKORNÁ, Catherine GAUTIER, J. UTILE et. al.

Základní údaje

Originální název

Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures

Název česky

Vazba ruznych monosacharidu lektinem PA-IIL z Pseudomonas aeruginosa: korelace termodynamickych dat s X-ray strukturami

Autoři

SABIN, Charles (250 Francie), Edward P. MITCHELL (826 Velká Británie a Severní Irsko), Martina POKORNÁ (203 Česká republika), Catherine GAUTIER (250 Francie), J. UTILE (250 Francie), Michaela WIMMEROVÁ (203 Česká republika, garant) a Anne IMBERTY (250 Francie)

Vydání

FEBS Letters, Amsterdam, Elsevier Science B.V. 2006, 0014-5793

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.372

Kód RIV

RIV/00216224:14310/06:00016740

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000235222500040

Klíčová slova anglicky

Pseudomonas aeruginosa; lectin; cystic fibrosis

Štítky

cystic fibrosis, lectin, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 1. 2007 15:32, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

ORIG CZ

V originále

The PA-IIL lectin from Pseudomonas aeruginosa is involved in host recognition and biofilm formation. The lectin displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. ITC experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-â-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. Combination of structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.

Česky

Vazba ruznych monosacharidu lektinem PA-IIL z Pseudomonas aeruginosa: korelace termodynamickych dat s X-ray strukturami

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

SABIN, Charles, Edward P. MITCHELL, Martina POKORNÁ, Catherine GAUTIER, J. UTILE, Michaela WIMMEROVÁ a Anne IMBERTY. Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures. FEBS Letters. Amsterdam: Elsevier Science B.V., 2006, roč. 580, č. 2, s. 982-987. ISSN 0014-5793.

@article{633505,
   author = {Sabin, Charles and Mitchell, Edward P. and Pokorná, Martina and Gautier, Catherine and Utile, J. and Wimmerová, Michaela and Imberty, Anne},
   article_location = {Amsterdam},
   article_number = {2},
   keywords = {Pseudomonas aeruginosa; lectin; cystic fibrosis},
   language = {eng},
   issn = {0014-5793},
   journal = {FEBS Letters},
   title = {Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures},
   volume = {580},
   year = {2006}
}

TY  - JOUR
ID  - 633505
AU  - Sabin, Charles - Mitchell, Edward P. - Pokorná, Martina - Gautier, Catherine - Utile, J. - Wimmerová, Michaela - Imberty, Anne
PY  - 2006
TI  - Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures
JF  - FEBS Letters
VL  - 580
IS  - 2
SP  - 982-987
EP  - 982-987
PB  - Elsevier Science B.V.
SN  - 00145793
KW  - Pseudomonas aeruginosa
KW  - lectin
KW  - cystic fibrosis
N2  - The PA-IIL lectin from Pseudomonas aeruginosa is involved in host recognition and biofilm formation. The lectin displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. ITC experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-â-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. Combination of structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.
ER  -

SABIN, Charles, Edward P. MITCHELL, Martina POKORNÁ, Catherine GAUTIER, J. UTILE, Michaela WIMMEROVÁ a Anne IMBERTY. Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures. \textit{FEBS Letters}. Amsterdam: Elsevier Science B.V., 2006, roč.~580, č.~2, s.~982-987. ISSN~0014-5793.

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