2006
Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
LOCHMAN, Jan a Vladimír MIKEŠZákladní údaje
Originální název
Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
Název česky
Ergosterol vyvolává expresi specifických obranných genů u tabáku
Autoři
LOCHMAN, Jan (203 Česká republika) a Vladimír MIKEŠ (203 Česká republika, garant)
Vydání
Plant Molecular Biology, UK, Springer, 2006, 0167-4412
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.577
Kód RIV
RIV/00216224:14310/06:00015653
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000240399300004
Klíčová slova anglicky
defence genes; Nicotiana tabacum; ergosterol; elicitor; salicylic acid; plant defence
Změněno: 18. 2. 2007 10:57, prof. RNDr. Vladimír Mikeš, CSc.
V originále
Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. Collected data shows that ergosterol is able to activate the expression of a number of defence genes and could increase resistance against pathogens.
Česky
Erosterol vyvolává expresi obranných genů u tabáku: PR1, PR3, PR5,PAL a kys. salicylové.
Návaznosti
| GA522/06/0156, projekt VaV |
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| MSM0021622413, záměr |
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