Electrochemical immunosensor for detection of Francisella
tularensis

C 2005

Electrochemical immunosensor for detection of Francisella tularensis

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ, Aleš MACELA et. al.

Základní údaje

Originální název

Electrochemical immunosensor for detection of Francisella tularensis

Název česky

Elektrochemický imunosensor pro detekci Francisella tularensis

Autoři

SKLÁDAL, Petr (203 Česká republika, garant), Yveta SYMERSKÁ (203 Česká republika), Miroslav POHANKA (203 Česká republika), Bohuslav ŠAFÁŘ (203 Česká republika) a Aleš MACELA (203 Česká republika)

Vydání

1. vyd. Dordrecht, The Netherlands, Defense against bioterror, od s. 221-232, 12 s. NATO Security through science ser. B, 2005

Nakladatel

Springer

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/05:00015144

Organizační jednotka

Přírodovědecká fakulta

ISBN

1-4020-3385-0

UT WoS

000236493200017

Klíčová slova anglicky

Biosensor; Biodetection; Biological agent

Štítky

Biodetection, Biological agent, biosensor

Změněno: 28. 4. 2006 12:47, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.

Česky

Byl vyvinut elektrochemický imunosensor pro rychlou detekci bakterie Francisella tularensis, patřící mezi biologické bojové prostředky.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ a Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In Defense against bioterror. 1. vyd. Dordrecht, The Netherlands: Springer, 2005, s. 221-232. NATO Security through science ser. B. ISBN 1-4020-3385-0.

@inbook{634237,
   author = {Skládal, Petr and Symerská, Yveta and Pohanka, Miroslav and Šafář, Bohuslav and Macela, Aleš},
   address = {Dordrecht, The Netherlands},
   booktitle = {Defense against bioterror},
   edition = {1},
   keywords = {Biosensor; Biodetection; Biological agent},
   language = {eng},
   location = {Dordrecht, The Netherlands},
   isbn = {1-4020-3385-0},
   pages = {221-232},
   publisher = {Springer},
   title = {Electrochemical immunosensor for detection of Francisella tularensis},
   year = {2005}
}

TY  - CHAP
ID  - 634237
AU  - Skládal, Petr - Symerská, Yveta - Pohanka, Miroslav - Šafář, Bohuslav - Macela, Aleš
PY  - 2005
TI  - Electrochemical immunosensor for detection of Francisella tularensis
VL  - NATO Security through science ser. B
PB  - Springer
CY  - Dordrecht, The Netherlands
SN  - 1402033850
KW  - Biosensor
KW  - Biodetection
KW  - Biological agent
N2  - The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
ER  -

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ a Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In \textit{Defense against bioterror}. 1. vyd. Dordrecht, The Netherlands: Springer, 2005, s.~221-232. NATO Security through science ser. B. ISBN~1-4020-3385-0.

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