d with electrochemical deUsing of flow injection analysis
coupletection for a determination of metallothionein

D 2006

d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM, René KIZEK et. al.

Základní údaje

Originální název

d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein

Název česky

Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu

Autoři

HORNA, Aleš (203 Česká republika), Radka MIKELOVÁ (203 Česká republika, garant), David POTĚŠIL (203 Česká republika), Vojtěch ADAM (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

Orlando, Florida, USA, 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006, od s. 430-22P, 1 s. 2006

Nakladatel

The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/06:00015699

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Flow injection analysis; electrochemical detection; metallothionein; tumor; cancer; metal ions;

Štítky

cancer, electrochemical detection, Flow injection analysis, metal ions, metallothionein, Tumor

Změněno: 18. 5. 2009 11:48, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. The MT overexpression is statistical significant at malignant and high grading tumours. Therefore it is necessary to suggest new methods for determination of MT. Electrochemical techniques enables to perform highly sensitive, low cost and easy-to-use determination of many biological active compounds. Here we studied the electrochemical behaviour of metallothionein by both stationary and flow system with electrochemical detection. The stationary electrochemical measurements (square wave voltammetry - SWV) were performed with the AUTOLAB Analyser using a standard cell with three electrodes, where the glassy carbon electrode served as a working electrode. Flow injection analysis with electrochemical detection (FIA-ED) was consisted from a chromatographic pump, a reaction coil and an electrochemical detector (Coulochem III) with glassy carbon electrode as a working electrode. Metallothionein gave signal at potential of 0.8 V using SWV. The SWV detection limit of MT was about units of micrograms per millilitre. In addition we performed the MT analysis using of FIA-ED. It was evident from the hydrodynamic voltammogram that the highest MT signal was obtained at detector potential of 0.8 V. In addition we studied relationship between overexpression of MT and progression of diseases in human tumours. MT overexpression would be a new prognostic marker of progression of tumours disease.

Česky

Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM a René KIZEK. d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein. In 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006. Orlando, Florida, USA: The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc., 2006, s. 430-22P, 1 s.

@inproceedings{636133,
   author = {Horna, Aleš and Mikelová, Radka and Potěšil, David and Adam, Vojtěch and Kizek, René},
   address = {Orlando, Florida, USA},
   booktitle = {57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006},
   keywords = {Flow injection analysis; electrochemical detection; metallothionein; tumor; cancer; metal ions;},
   language = {eng},
   location = {Orlando, Florida, USA},
   pages = {430-22P},
   publisher = {The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.},
   title = {d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein},
   year = {2006}
}

TY  - JOUR
ID  - 636133
AU  - Horna, Aleš - Mikelová, Radka - Potěšil, David - Adam, Vojtěch - Kizek, René
PY  - 2006
TI  - d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein
PB  - The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.
CY  - Orlando, Florida, USA
KW  - Flow injection analysis
KW  - electrochemical detection
KW  - metallothionein
KW  - tumor
KW  - cancer
KW  - metal ions;
N2  - The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. The MT overexpression is statistical significant at malignant and high grading tumours. Therefore it is necessary to suggest new methods for determination of MT. Electrochemical techniques enables to perform highly sensitive, low cost and easy-to-use determination of many biological active compounds. Here we studied the electrochemical behaviour of metallothionein by both stationary and flow system with electrochemical detection. The stationary electrochemical measurements (square wave voltammetry - SWV) were performed with the AUTOLAB Analyser using a standard cell with three electrodes, where the glassy carbon electrode served as a working electrode. Flow injection analysis with electrochemical detection (FIA-ED) was consisted from a chromatographic pump, a reaction coil and an electrochemical detector (Coulochem III) with glassy carbon electrode as a working electrode. Metallothionein gave signal at potential of 0.8 V using SWV. The SWV detection limit of MT was about units of micrograms per millilitre. In addition we performed the MT analysis using of FIA-ED. It was evident from the hydrodynamic voltammogram that the highest MT signal was obtained at detector potential of 0.8 V. In addition we studied relationship between overexpression of MT and progression of diseases in human tumours. MT overexpression would be a new prognostic marker of progression of tumours disease.
ER  -

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM a René KIZEK. d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein. In \textit{57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006}. Orlando, Florida, USA: The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc., 2006, s.~430-22P, 1 s.

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