Role of hemolymph factors during in vitro cellular encapsulation of nematodes by the hemocytes of Galleria mellonella

NALINI, Madanagopal, Pavel HYRŠL and Thiagarajan RAMAN. Role of hemolymph factors during in vitro cellular encapsulation of nematodes by the hemocytes of Galleria mellonella. In Summit Workshop Cost Actions 850 "Biocontrol Symbiosis". Salzau, Germany: Ralf-Udo Ehlers, 2006, p. 56-56.

Basic information

Original name

Role of hemolymph factors during in vitro cellular encapsulation of nematodes by the hemocytes of Galleria mellonella

Authors

NALINI, Madanagopal, Pavel HYRŠL and Thiagarajan RAMAN

Edition

Salzau, Germany, Summit Workshop Cost Actions 850 "Biocontrol Symbiosis" p. 56-56, 1 pp. 2006

Publisher

Ralf-Udo Ehlers

---

Other information

Type of outcome

Stať ve sborníku

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

---

Abstract

In English

The anticoagulant buffer used to collect hemolymph in Galleria mellonella, enabled the identification of five different hemocyte morphotypes with a viability of >95% and these hemocytes encapsulated Heterorhabditis bacteriophora both in vivo and in vitro. Compared to in vivo and in vitro conditions, the entomopathogenic nematodes were encapsulated at a higher rate only under in vitro conditions. The per cent encapsulation response was altered by the presence or absence of various hemolymph factors namely, plasma, hemocyte lysate supernatant and hemocyte conditioned medium influenced the encapsualtion response of these hemocytes. The molecules present in the hemocyte conditioned medium identified using SDS-PAGE, appear to regulate the cellular encapsulation response.