A suggestion of enzymatic biosensor for determination of
chlorinated pesticides

D 2006

A suggestion of enzymatic biosensor for determination of chlorinated pesticides

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM et. al.

Základní údaje

Originální název

A suggestion of enzymatic biosensor for determination of chlorinated pesticides

Název česky

Návrh enzymatického biosenzoru pro detekci chlorovaných pesticidů

Autoři

MIKELOVÁ, Radka (203 Česká republika), Zbyněk PROKOP (203 Česká republika), Jan HRADECKÝ (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant), Vojtěch ADAM (203 Česká republika), Jiří DAMBORSKÝ (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

první. Bordeaux, 11th International conference on electroanalysis, od s. 1-45, 300 s. 2006

Nakladatel

European Society for ElectroAnalytical Chemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/06:00020011

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

silver electrode; carbon past electrode; modification; chloride; chlorinated hydrocarbon; electrochemistry

Štítky

carbon past electrode, chloride, chlorinated hydrocarbon, electrochemistry, Modification, silver electrode

Změněno: 26. 7. 2007 16:20, prof. Mgr. Jiří Damborský, Dr.

Anotace

ORIG CZ

V originále

Introduction. Chlorinated pesticides such as derivates of DTT, hexachlorobenzene and many others have been used to protect of plants. It is a common knowledge that residues of these pesticides deposit in adipose tissues at animals and could be transferred from here to eggs or to breast milk of mammals. A higher concentration of these compounds could be responsible for infertility and for tumour disease. Therefore a development of simple and accurate tool for detection of them is need. Objective. The aim of this work was to suggest a biosensor used for determination of chlorinated pesticides. Materials and methods. Stationary electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was carbon paste electrode. A flow injection analysis coupled with electrochemical detector consisted of solvent delivery pump, a guard cell, a reaction coil (1 m) and an electrochemical detector. The electrochemical detector includes one low volume flow-through analytical cells, which is consisted of glassy carbon working electrode, palladium electrode as reference electrode and auxiliary carbon electrode, and Coulochem III as a control module. Results. Our suggested biosensor is based on a determination of chlorides on carbon paste and/or glassy carbon electrode both in stationary and flow system. We improved sensitivity and selectivity of the analysis by stationary electrochemical system, particularly by carbon past electrode, through modification of the electrode by silver nitrate. We were able to determine chlorides in units of 10-8 M due to this modification. After that we evaluated that we were able to determine chloride, we suggested our biosensor, which were consisted from the electrode and a dehalogenases. The enzymes were capable of dechlorinating chlorinated hydrocarbons. We applied the biosensor to detect a 1-chlorohexane and were successful. Moreover, we attempted to determine chlorinated hydrocarbons by flow technique with electrochemical detection. Conclusions. Commonly used methods for determination of chlorinated pesticides are time consuming and highly cost. Here, we suggested a technique for simple and low cost determination of these compounds.

Česky

Návrh enzymatického biosenzoru pro detekci chlorovaných pesticidů

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM, Jiří DAMBORSKÝ a René KIZEK. A suggestion of enzymatic biosensor for determination of chlorinated pesticides. In 11th International conference on electroanalysis. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, s. 1-45, 300 s.

@inproceedings{637914,
   author = {Mikelová, Radka and Prokop, Zbyněk and Hradecký, Jan and Trnková, Libuše and Adam, Vojtěch and Damborský, Jiří and Kizek, René},
   address = {Bordeaux},
   booktitle = {11th International conference on electroanalysis},
   edition = {první},
   keywords = {silver electrode; carbon past electrode; modification; chloride; chlorinated hydrocarbon; electrochemistry},
   language = {eng},
   location = {Bordeaux},
   pages = {1-45},
   publisher = {European Society for ElectroAnalytical Chemistry},
   title = {A suggestion of enzymatic biosensor for determination of chlorinated pesticides},
   year = {2006}
}

TY  - JOUR
ID  - 637914
AU  - Mikelová, Radka - Prokop, Zbyněk - Hradecký, Jan - Trnková, Libuše - Adam, Vojtěch - Damborský, Jiří - Kizek, René
PY  - 2006
TI  - A suggestion of enzymatic biosensor for determination of chlorinated pesticides
PB  - European Society for ElectroAnalytical Chemistry
CY  - Bordeaux
KW  - silver electrode
KW  - carbon past electrode
KW  - modification
KW  - chloride
KW  - chlorinated hydrocarbon
KW  - electrochemistry
N2  - Introduction. Chlorinated pesticides such as derivates of DTT, hexachlorobenzene and many others have been used to protect of plants. It is a common knowledge that residues of these pesticides deposit in adipose tissues at animals and could be transferred from here to eggs or to breast milk of mammals. A higher concentration of these compounds could be responsible for infertility and for tumour disease. Therefore a development of simple and accurate tool for detection of them is need. Objective. The aim of this work was to suggest a biosensor used for determination of chlorinated pesticides. Materials and methods. Stationary electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was carbon paste electrode. A flow injection analysis coupled with electrochemical detector consisted of solvent delivery pump, a guard cell, a reaction coil (1 m) and an electrochemical detector. The electrochemical detector includes one low volume flow-through analytical cells, which is consisted of glassy carbon working electrode, palladium electrode as reference electrode and auxiliary carbon electrode, and Coulochem III as a control module. Results. Our suggested biosensor is based on a determination of chlorides on carbon paste and/or glassy carbon electrode both in stationary and flow system. We improved sensitivity and selectivity of the analysis by stationary electrochemical system, particularly by carbon past electrode, through modification of the electrode by silver nitrate. We were able to determine chlorides in units of 10-8 M due to this modification. After that we evaluated that we were able to determine chloride, we suggested our biosensor, which were consisted from the electrode and a dehalogenases. The enzymes were capable of dechlorinating chlorinated hydrocarbons. We applied the biosensor to detect a 1-chlorohexane and were successful. Moreover, we attempted to determine chlorinated hydrocarbons by flow technique with electrochemical detection. Conclusions. Commonly used methods for determination of chlorinated pesticides are time consuming and highly cost. Here, we suggested a technique for simple and low cost determination of these compounds.
ER  -

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM, Jiří DAMBORSKÝ a René KIZEK. A suggestion of enzymatic biosensor for determination of chlorinated pesticides. In \textit{11th International conference on electroanalysis}. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, s.~1-45, 300 s.

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