An analysis of tissues of vertebrates treated by Bromadiolone

D 2006

An analysis of tissues of vertebrates treated by Bromadiolone

KŘÍŽKOVÁ, Soňa, Veronika ŠUPÁKOVÁ, Radka MIKELOVÁ, Vojtěch ADAM, Miroslava BEKLOVÁ et. al.

Základní údaje

Originální název

An analysis of tissues of vertebrates treated by Bromadiolone

Název česky

Analýza tkání z hrabošů krmených bromadiolonem

Autoři

KŘÍŽKOVÁ, Soňa (203 Česká republika), Veronika ŠUPÁKOVÁ (203 Česká republika), Radka MIKELOVÁ (203 Česká republika, garant), Vojtěch ADAM (203 Česká republika), Miroslava BEKLOVÁ (203 Česká republika), J. PIKULA (203 Česká republika), Michal SVOBODA (203 Česká republika), Aleě HORNA (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

prvn. Trencianske Teplice, Toxcon 2006; 11. Interdisciplinary Slovak - Czech Toxicological Conference, od s. 39-39, 300 s. 2006

Nakladatel

Slovak Toxicology Society SETOX

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/06:00015746

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-969474-1-9

Klíčová slova anglicky

Electrochemistry; Bromadiolone; Lanirat; HPLC-ED; glassy carbon electrode; cyclic voltammetry;

Štítky

Bromadiolone, cyclic voltammetry, electrochemistry, glassy carbon electrode, HPLC-ED, Lanirat

Změněno: 29. 6. 2006 15:50, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

Bromadiolone is a 4-hydroxy-coumarine derivative used as rodenticide, which shows an anticoagulant effect based on the prothrombine synthesis inhibition. The determination of this compound has interest in toxicological analysis owing to its high accute oral toxicity. The dose of bromadiolone, which is expected to cause death of 50 % of male rats by oral administration (LD50) is 1.125 mg.kg-1. A determination of this compound of interest has also interest in environmental analysis taking into account its wide use to control rodent populations, which involves the resulting risk of water pollution or intoxication of non-target species. Thus we focused on suggesting of a new technique for determination of bromadiolone using high-performance liquid chromatography with electrochemical detection (HPLC-ED). Prior to analysis in flow system we have studied the basic electrochemical behaviour of bromadiolone by cyclic voltammetry on Autolab analyser (EcoChemie, Netherlands) connected with VA-Stand 663 (Metrohm, Schwitzerland). On the base of experimental results from cyclic voltammetry we designed optimal conditions for flow analysis of bromadiolone. Our analysis by high-performance liquid chromatography was carried out on CoulochemIII analyser (ESA, Inc.). Hydrogenpaladium electrode, auxiliary carbon electrode and working glassy carbon electrode were placed in electrochemical cell of volume of 50 ľl referent. We used acetate buffer (pH = 4.0):acetonitrile in ratio of 40/60 (v/v) as the most suitable mobile phase for determination of the compound of interest. We construed a hydrodynamic voltammogram, which is a dependence of peak height on applied potential. It clearly follows from the dependence that the most suitable potential for determination of bromadiolone is 836 mV. We found out that our method is sensitive enough for analysis of real samples because allows us to determine tens of nanograms of bromadiolone per ml. We utilized this optimized method to determine the amount of bromadiolone in real samples of tissues of animals which eaten bromadiolone in different forms. Earthworms (Eisenia fetida), were kept in presence of five granules of rodenticide Lanirat G in five litres of artificial soil (88 ěg/l). These earthworms were used as a feed for field mice (Microtus arvalis). Other group of field mice was fed by defined amount of bromadiolone. Earthworms kept without rodenticide and field mice fed with non-treated earthworms or fed by usual feed for rodents served as a control. We analysed the content of bromadiolone in liver of these field mice. Then we applied this method to determine the bromadiolone content in liver and stomach content of three hares (Lepus europaeus), which were found death in area where bromadiolone was applied. We found out that one them died by bromadiolone. The suggested technique is fast, sensitive and allows us to determine a trace amount of bromadiolone in biological matrix. Moreover we are able to monitor the incidence of using of bromadiolone on non target fauna.

Česky

Analýza tkání z hrabošů krmených bromadiolonem

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

KŘÍŽKOVÁ, Soňa, Veronika ŠUPÁKOVÁ, Radka MIKELOVÁ, Vojtěch ADAM, Miroslava BEKLOVÁ, J. PIKULA, Michal SVOBODA, Aleě HORNA a René KIZEK. An analysis of tissues of vertebrates treated by Bromadiolone. In Toxcon 2006; 11. Interdisciplinary Slovak - Czech Toxicological Conference. prvn. Trencianske Teplice: Slovak Toxicology Society SETOX, 2006, s. 39-39, 300 s. ISBN 80-969474-1-9.

@inproceedings{638528,
   author = {Křížková, Soňa and Šupáková, Veronika and Mikelová, Radka and Adam, Vojtěch and Beklová, Miroslava and Pikula, J. and Svoboda, Michal and Horna, Aleě and Kizek, René},
   address = {Trencianske Teplice},
   booktitle = {Toxcon 2006; 11. Interdisciplinary Slovak - Czech Toxicological Conference},
   edition = {prvn},
   keywords = {Electrochemistry; Bromadiolone; Lanirat; HPLC-ED; glassy carbon electrode; cyclic voltammetry;},
   language = {eng},
   location = {Trencianske Teplice},
   isbn = {80-969474-1-9},
   pages = {39-39},
   publisher = {Slovak Toxicology Society SETOX},
   title = {An analysis of tissues of vertebrates treated by Bromadiolone},
   year = {2006}
}

TY  - JOUR
ID  - 638528
AU  - Křížková, Soňa - Šupáková, Veronika - Mikelová, Radka - Adam, Vojtěch - Beklová, Miroslava - Pikula, J. - Svoboda, Michal - Horna, Aleě - Kizek, René
PY  - 2006
TI  - An analysis of tissues of vertebrates treated by Bromadiolone
PB  - Slovak Toxicology Society SETOX
CY  - Trencianske Teplice
SN  - 8096947419
KW  - Electrochemistry
KW  - Bromadiolone
KW  - Lanirat
KW  - HPLC-ED
KW  - glassy carbon electrode
KW  - cyclic voltammetry;
N2  - Bromadiolone is a 4-hydroxy-coumarine derivative used as rodenticide, which shows an anticoagulant effect based on the prothrombine synthesis inhibition. The determination of this compound has interest in toxicological analysis owing to its high accute oral toxicity. The dose of bromadiolone, which is expected to cause death of 50 % of male rats by oral administration (LD50) is 1.125 mg.kg-1. A determination of this compound of interest has also interest in environmental analysis taking into account its wide use to control rodent populations, which involves the resulting risk of water pollution or intoxication of non-target species. Thus we focused on suggesting of a new technique for determination of bromadiolone using high-performance liquid chromatography with electrochemical detection (HPLC-ED). Prior to analysis in flow system we have studied the basic electrochemical behaviour of bromadiolone by cyclic voltammetry on Autolab analyser (EcoChemie, Netherlands) connected with VA-Stand 663 (Metrohm, Schwitzerland). On the base of experimental results from cyclic voltammetry we designed optimal conditions for flow analysis of bromadiolone. Our analysis by high-performance liquid chromatography was carried out on CoulochemIII analyser (ESA, Inc.). Hydrogenpaladium electrode, auxiliary carbon electrode and working glassy carbon electrode were placed in electrochemical cell of volume of 50 ľl referent. We used acetate buffer (pH = 4.0):acetonitrile in ratio of 40/60 (v/v) as the most suitable mobile phase for determination of the compound of interest. We construed a hydrodynamic voltammogram, which is a dependence of peak height on applied potential. It clearly follows from the dependence that the most suitable potential for determination of bromadiolone is 836 mV. We found out that our method is sensitive enough for analysis of real samples because allows us to determine tens of nanograms of bromadiolone per ml. We utilized this optimized method to determine the amount of bromadiolone in real samples of tissues of animals which eaten bromadiolone in different forms. Earthworms (Eisenia fetida), were kept in presence of five granules of rodenticide Lanirat G in five litres of artificial soil (88 ěg/l). These earthworms were used as a feed for field mice (Microtus arvalis). Other group of field mice was fed by defined amount of bromadiolone. Earthworms kept without rodenticide and field mice fed with non-treated earthworms or fed by usual feed for rodents served as a control. We analysed the content of bromadiolone in liver of these field mice. Then we applied this method to determine the bromadiolone content in liver and stomach content of three hares (Lepus europaeus), which were found death in area where bromadiolone was applied. We found out that one them died by bromadiolone. The suggested technique is fast, sensitive and allows us to determine a trace amount of bromadiolone in biological matrix. Moreover we are able to monitor the incidence of using of bromadiolone on non target fauna.
ER  -

KŘÍŽKOVÁ, Soňa, Veronika ŠUPÁKOVÁ, Radka MIKELOVÁ, Vojtěch ADAM, Miroslava BEKLOVÁ, J. PIKULA, Michal SVOBODA, Aleě HORNA a René KIZEK. An analysis of tissues of vertebrates treated by Bromadiolone. In \textit{Toxcon 2006; 11. Interdisciplinary Slovak - Czech Toxicological Conference}. prvn. Trencianske Teplice: Slovak Toxicology Society SETOX, 2006, s.~39-39, 300 s. ISBN~80-969474-1-9.

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