Detailed Information on Publication Record
2006
MAPPING AND IDENTIFICATION OF POWDERY MILDEW RESISTANCE GENES IN A NEW ACCESSION OF HORDEUM VULGARE SSP. SPONTANEUM
TETUROVÁ, KateřinaBasic information
Original name
MAPPING AND IDENTIFICATION OF POWDERY MILDEW RESISTANCE GENES IN A NEW ACCESSION OF HORDEUM VULGARE SSP. SPONTANEUM
Name in Czech
Mapování a identifikace genů rezistence k padlí travnímu u nového zdroje rezistence Hordeum vulgare ssp. spontaneum
Authors
TETUROVÁ, Kateřina (203 Czech Republic, guarantor)
Edition
MU Brno, X. Pracovní setkání biochemiků a molekulárních biologů - Sborník příspěvků, p. 43-43, 2006
Publisher
Vydavatelství MU Brno
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/06:00015773
Organization unit
Faculty of Science
ISBN
80-210-3942-6
Keywords in English
barley; powdery mildew; resistance genes; DNA markers
Změněno: 25/7/2006 10:53, Ing. Kateřina Teturová, Ph.D.
V originále
The genetic analysis of a newly identified accession of wild barley (Hordeum vulgare ssp. spontaneum) resistant to powdery mildew (Blumeria graminis f. sp. hordei) showed that the resistance was determined by two dominant genes. The aim of the molecular analysis was to localize the resistance genes on barley genetic map using DNA markers (SSRs mostly). The analyses were performed in the F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. The susceptible F2 plants were screened to identify the markers linked with the resistance genes. Data from F2 plants of all genotypes were analysed in the MapManager QTX program to confirm the linkage and to assign the order and the distances between the markers. The molecular analysis revealed a highly significant linkage with Bmac0213 and MGB402 on the chromosome 1H, which is the position consistent with the Mla locus. The other gene was proved to be linked with Bmac0134 and MWG878 on the short arm of chromosome 2H. The prospect of the work is to find more tightly linked DNA markers so that breeders could use them for marker assisted selection.
In Czech
U nově identifikovaného zdroje rezistence PI466197 (Hordeum vulgare ssp. spontaneum) k padlí travnímu (Blumeria graminis f. sp. hordei) byla genetickou analýzou stanovena přítomnost dvou dominantních genů rezistence, z nichž jeden se nachází v lokusu Mla. Molekulární analýzou pomocí DNA markerů byla určena pozice jednoho genu na krátkém rameni chromozomu 1H mezi markery Bmac0213 a MGB402 (oblast lokusu Mla) a druhého genu na krátkém rameni chromozomu 2H mezi markery Bmac0134 a MWG878.
Links
| GA522/03/0112, research and development project |
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| GD204/05/H505, research and development project |
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