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@article{695967,
author = {Nováková, Dana and Pantůček, Roman and Petráš, Petr and Koukalová, Dagmar and Sedláček, Ivo},
article_location = {United Kingdom},
article_number = {2},
keywords = {Staphylococcus nepalensis; coagulase-negative staphylococci; ribotyping; whole-cell protein analysis; macrorestriction analysis; 16S rRNA gene sequencing},
language = {eng},
issn = {0378-1097},
journal = {FEMS Microbiology Letters},
title = {Occurance of Staphylococcus nepalensis strains in different sources including human clinical material},
url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6968.2006.00408.x},
volume = {263},
year = {2006}
}
TY - JOUR
ID - 695967
AU - Nováková, Dana - Pantůček, Roman - Petráš, Petr - Koukalová, Dagmar - Sedláček, Ivo
PY - 2006
TI - Occurance of Staphylococcus nepalensis strains in different sources including human clinical material
JF - FEMS Microbiology Letters
VL - 263
IS - 2
SP - 163-168
EP - 163-168
PB - Blackwell Publishing Ltd.
SN - 03781097
KW - Staphylococcus nepalensis
KW - coagulase-negative staphylococci
KW - ribotyping
KW - whole-cell protein analysis
KW - macrorestriction analysis
KW - 16S rRNA gene sequencing
UR - http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6968.2006.00408.x
N2 - Five isolates of coagulase-negative staphylococci were obtained from human urine, the gastrointestinal tract of squirrel monkeys, pig skin and from the environment. All key biochemical characteristics of the tested strains corresponded with the description of Staphylococcus xylosus species. However, partial 16S rRNA gene sequences obtained from analysed strains corresponded with those of Staphylococcus nepalensis reference strains, except for two strains which differed in one residue. Ribotyping with EcoRI and HindIII restriction enzymes, whole cell protein profile analysis performed by SDS-PAGE and SmaI macrorestriction analysis were used for more precise characterization and identification of the analysed strains. Obtained results showed that EcoRI and HindIII ribotyping and whole cell protein fingerprinting are suitable and reliable methods for the differentiation of S. nepalensis strains from the other novobiocin resistant staphylococci, whereas macrorestriction analysis was found to be a good tool for strain typing. The isolation of S. nepalensis is sporadic, and according to our best knowledge this study is the first report of the occurrence of this species in human clinical material as well as in other sources.
ER -
NOVÁKOVÁ, Dana, Roman PANTŮČEK, Petr PETRÁŠ, Dagmar KOUKALOVÁ a Ivo SEDLÁČEK. Occurance of $<$I$>$Staphylococcus nepalensis$<$/I$>$ strains in different sources including human clinical material. \textit{FEMS Microbiology Letters}. United Kingdom: Blackwell Publishing Ltd., 2006, roč.~263, č.~2, s.~163-168. ISSN~0378-1097.
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