KOCÁBEK, Tomáš, Jana ŘEPKOVÁ, Markéta DUDOVÁ, Klára HOYEROVÁ a Lukáš VRBA. Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein. Genetica. Nizozemí: Springer Netherlands, 2006, roč. 128, 1-3, s. 395-407. ISSN 0016-6707. |
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@article{698268, author = {Kocábek, Tomáš and Řepková, Jana and Dudová, Markéta and Hoyerová, Klára and Vrba, Lukáš}, article_location = {Nizozemí}, article_number = {1-3}, keywords = {Arabidopsis thaliana - embryonic defect - mitochondria - pentatricopeptide repeat - semi-lethality - T-DNA mutagenesis}, language = {eng}, issn = {0016-6707}, journal = {Genetica}, title = {Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein}, volume = {128}, year = {2006} }
TY - JOUR ID - 698268 AU - Kocábek, Tomáš - Řepková, Jana - Dudová, Markéta - Hoyerová, Klára - Vrba, Lukáš PY - 2006 TI - Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein JF - Genetica VL - 128 IS - 1-3 SP - 395-407 EP - 395-407 PB - Springer Netherlands SN - 00166707 KW - Arabidopsis thaliana - embryonic defect - mitochondria - pentatricopeptide repeat - semi-lethality - T-DNA mutagenesis N2 - A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis comfirmed that phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow. ER -
KOCÁBEK, Tomáš, Jana ŘEPKOVÁ, Markéta DUDOVÁ, Klára HOYEROVÁ a Lukáš VRBA. Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein. \textit{Genetica}. Nizozemí: Springer Netherlands, 2006, roč.~128, 1-3, s.~395-407. ISSN~0016-6707.
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