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@article{700001,
author = {Paleček, Jan and Vidot, Susanne and Feng, Min and Doherty, Aidan and Lehmann, Alan},
article_number = {48},
keywords = {SMC complex, SMC5/6 architecture, kleisin subunit,},
journal = {Journal of Biological Chemistry},
title = {The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17005570&query_hl=1&itool=pubmed_docsum},
volume = {281},
year = {2006}
}
TY - JOUR
ID - 700001
AU - Paleček, Jan - Vidot, Susanne - Feng, Min - Doherty, Aidan - Lehmann, Alan
PY - 2006
TI - The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...
JF - Journal of Biological Chemistry
VL - 281
IS - 48
SP - 36952-9
EP - 36952-9
KW - SMC complex, SMC5/6 architecture, kleisin subunit,
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17005570&query_hl=1&itool=pubmed_docsum
N2 - Structural maintenance of chromosomes (SMC) proteins play fundamental roles in many aspects of chromosome organization and dynamics. The SMC complexes form unique structures with long coiled-coil arms folded at a hinge domain, so that the globular N- and C-terminal domains are brought together to form a "head." Within the Smc5-Smc6 complex, we previously identified two subcomplexes containing Smc6-Smc5-Nse2 and Nse1-Nse3-Nse4. A third subcomplex containing Nse5 and -6 has also been identified recently. We present evidence that Nse4 is the kleisin component of the complex, which bridges the heads of Smc5 and -6. The C-terminal part of Nse4 interacts with the head domain of Smc5, and structural predictions for Nse4 proteins suggest similar motifs that are shared within the kleisin family. Specific mutations within a predicted winged helix motif of Nse4 destroy the interaction with Smc5. We propose that Nse4 and its orthologs form the delta-kleisin subfamily. We further show that Nse3, as well as Nse5 and Nse6, also bridge the heads of Smc5 and -6. The Nse1-Nse3-Nse4 and Nse5-Nse6 subcomplexes bind to the Smc5-Smc6 heads domain at different sites.
ER -
PALEČEK, Jan, Susanne VIDOT, Min FENG, Aidan DOHERTY and Alan LEHMANN. The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ... \textit{Journal of Biological Chemistry}. 2006, vol.~281, No~48, p.~36952-9.
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