2007
Phylogenetic Analysis of Haloalkane Dehalogenases
CHOVANCOVÁ, Eva, Jan KOSINSKI, Janusz M. BUJNICKI a Jiří DAMBORSKÝZákladní údaje
Originální název
Phylogenetic Analysis of Haloalkane Dehalogenases
Název česky
Fytogenetická analýza halogenalkandehalogenáz
Autoři
CHOVANCOVÁ, Eva (203 Česká republika), Jan KOSINSKI (616 Polsko), Janusz M. BUJNICKI (616 Polsko) a Jiří DAMBORSKÝ (203 Česká republika, garant)
Vydání
Proteins: Structure, Function, and Bioinformatics, 2007, 0887-3585
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.354
Kód RIV
RIV/00216224:14310/07:00021728
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000245021800006
Klíčová slova anglicky
Haloalkane dehalogenases; biochemical analyses; phylogenetic and functional relationships;
Příznaky
Recenzováno
Změněno: 19. 3. 2010 12:25, prof. Mgr. Jiří Damborský, Dr.
V originále
Haloalkane dehalogenases (HLDs) are enzymes that catalyze the cleavage of carbon halogen bonds by a hydrolytic mechanism. Although comparative biochemical analyses have been published, no classification system has been proposed for HLDs, to date, that reconciles their phylogenetic and functional relationships. In the study presented here we have analyzed all sequences and structures of genuine HLDs and their homologs detectable by database searches. Phylogenetic analyses revealed that the HLD family can be divided into three subfamilies denoted HLD-I, HLD-II and HLD-III, of which HLD-I and HLD-III are predicted to be sister groups. A mismatch between the HLD protein tree and the tree of species, as well as the presence of more than one HLD gene in a few genomes, suggest that horizontal gene transfers, and perhaps also multiple gene duplications and losses have been involved in the evolution of this family. Most of the biochemically characterized HLDs are found in the HLD-II subfamily. The dehalogenating activity of two members of the newly identified HLD-III subfamily has only recently been confirmed, in a study motivated by this phylogenetic analysis. A novel type of the catalytic pentad (Asp-His-Asp+Asn-Trp) was predicted for members of the HLD-III subfamily. Calculation of the evolutionary rates and lineage-specific innovations revealed a common conserved core as well as a set of residues that characterizes each HLD subfamily. The N-terminal part of the cap domain is one of the most variable regions within the whole family as well as within individual subfamilies, and serves as a preferential site for the location of relatively long insertions. The highest variability of discrete sites was observed among residues that are structural components of the access channels. Mutations at these sites modify the anatomy of the channels, which are important for the exchange of ligands between the buried active site and the bulk solvent, thus creating a structural basis for the molecular evolution of new substrate specificities. Our analysis sheds light on the evolutionary history of HLDs and provides a structural framework for designing enzymes with new specificities.
Česky
Halogenalkandehalogenázy (HLDs)jsou enzymy katalyzující hydrolytické štěpení vazby uhlík-halogen. V článku je publikován klasifikační systém pro HLDs, byly analyzovány všechny dostupné sekvence a struktury HLDs a jejich homologů, ............
Návaznosti
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