KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. Microbiology-SGM. Reading, Berks (Great Britain): Society for General Microbiology, 2006, vol. 152, No 12, p. 3529-3534. ISSN 1350-0872.
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Basic information
Original name Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans
Name in Czech Ovlivnění redukce dusičnanu v nerostoucích buňkách Paracoccus denitrificans chlorečnanem a chloritanem
Authors KUČERA, Igor (203 Czech Republic, guarantor).
Edition Microbiology-SGM, Reading, Berks (Great Britain), Society for General Microbiology, 2006, 1350-0872.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.139
RIV identification code RIV/00216224:14310/06:00017489
Organization unit Faculty of Science
UT WoS 000243205800007
Keywords in English nitrate respiration; nitrate reductase; chlorate; chlorite; enzyme inactivation
Tags chlorate, chlorite, enzyme inactivation, nitrate reductase, nitrate respiration
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Igor Kučera, DrSc., učo 911. Changed: 16/2/2007 07:07.
Abstract
When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth on butyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite. Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells and limited permeation of chlorite across the cell membrane.
Abstract (in Czech)
Při anaerobním růstu na mediu se sukcinátem a nitrátem (SN) vytváří Paracoccus denitrificans membránově vázanou nitrátreduktasu Nar, zatímco při aerobním růstu na butyrátu (BO) vzniká periplazmatický enzym Nap. Preinkubace SN buněk s chlorečnanem vedla k inaktivaci Nar. Toluenizace snižovala citlivost Nar k chlorečnanu a zvyšovala citlivost k chloritanu. Nap v BO buňkách byla inaktivována již o tři řády nižšími koncentracemi chlorečnanu i chloritanu. Částečně purifikovaná Nap byla necitlivá k chlorečnanu a méně citlivá k chloritanu. Nar před působením chlorečnanu specificky chránil azid, Nap dusičnan. Výsledky svědčí o rozdílné metabolické aktivaci oxoaniontů Cl v obou typech buněk a o omezené propustnosti buněčné membrány pro chloritan.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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