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@inproceedings{701551,
author = {Šulák, Ondřej and Spanilá, Miroslava and Gajdošová, Dagmar and Pazourek, Jiří and Havel, Josef},
address = {Prague, Czech republic},
booktitle = {Collection Symposium Series, Biologically Active Peptides},
keywords = {Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide},
language = {eng},
location = {Prague, Czech republic},
isbn = {8086241262},
pages = {87-90},
publisher = {Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic},
title = {Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides},
year = {2005}
}
TY - JOUR
ID - 701551
AU - Šulák, Ondřej - Spanilá, Miroslava - Gajdošová, Dagmar - Pazourek, Jiří - Havel, Josef
PY - 2005
TI - Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
PB - Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic
CY - Prague, Czech republic
SN - 8086241262
KW - Capillary electrophoresis
KW - Coating
KW - Electroosmotic flow
KW - Polyethyleneimine
KW - 3glycidoxypropyltrimethoxysilane
KW - 3aminopropyltriethoxysilane
KW - Linear polyacrylamide
N2 - Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
ER -
ŠULÁK, Ondřej, Miroslava SPANILÁ, Dagmar GAJDOŠOVÁ, Jiří PAZOUREK and Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In \textit{Collection Symposium Series, Biologically Active Peptides}. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p.~87-90. ISBN~8086241262.
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