Minimization of adsorption in capillary zone electrophoresis of
proteins and neuroprotective peptides

D 2005

Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides

ŠULÁK, Ondřej, Miroslava SPANILÁ, Dagmar GAJDOŠOVÁ, Jiří PAZOUREK, Josef HAVEL et. al.

Basic information

Original name

Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides

Name in Czech

Minimalizace adsorpce proteinů a neuroprotektivních peptidů v kapilární zónové elektroforéze

Authors

ŠULÁK, Ondřej (203 Czech Republic, guarantor), Miroslava SPANILÁ (203 Czech Republic), Dagmar GAJDOŠOVÁ (203 Czech Republic), Jiří PAZOUREK (203 Czech Republic) and Josef HAVEL (203 Czech Republic)

Edition

Prague, Czech republic, Collection Symposium Series, Biologically Active Peptides, p. 87-90, 4 pp. 2005

Publisher

Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00020042

Organization unit

Faculty of Science

ISBN

8086241262

Keywords in English

Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide

Abstract

ORIG CZ

V originále

Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.

In Czech

Links

GA305/03/1100, research and development project

Name: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu

Citovat

ŠULÁK, Ondřej, Miroslava SPANILÁ, Dagmar GAJDOŠOVÁ, Jiří PAZOUREK and Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In Collection Symposium Series, Biologically Active Peptides. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p. 87-90. ISBN 8086241262.

@inproceedings{701551,
   author = {Šulák, Ondřej and Spanilá, Miroslava and Gajdošová, Dagmar and Pazourek, Jiří and Havel, Josef},
   address = {Prague, Czech republic},
   booktitle = {Collection Symposium Series, Biologically Active Peptides},
   keywords = {Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide},
   language = {eng},
   location = {Prague, Czech republic},
   isbn = {8086241262},
   pages = {87-90},
   publisher = {Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic},
   title = {Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides},
   year = {2005}
}

TY  - JOUR
ID  - 701551
AU  - Šulák, Ondřej - Spanilá, Miroslava - Gajdošová, Dagmar - Pazourek, Jiří - Havel, Josef
PY  - 2005
TI  - Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
PB  - Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic
CY  - Prague, Czech republic
SN  - 8086241262
KW  - Capillary electrophoresis
KW  - Coating
KW  - Electroosmotic flow
KW  - Polyethyleneimine
KW  - 3glycidoxypropyltrimethoxysilane
KW  - 3aminopropyltriethoxysilane
KW  - Linear polyacrylamide
N2  - Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
ER  -

ŠULÁK, Ondřej, Miroslava SPANILÁ, Dagmar GAJDOŠOVÁ, Jiří PAZOUREK and Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In \textit{Collection Symposium Series, Biologically Active Peptides}. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p.~87-90. ISBN~8086241262.

Detailed Information on Publication Record