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@inproceedings{701551, author = {Šulák, Ondřej and Spanilá, Miroslava and Gajdošová, Dagmar and Pazourek, Jiří and Havel, Josef}, address = {Prague, Czech republic}, booktitle = {Collection Symposium Series, Biologically Active Peptides}, keywords = {Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide}, language = {eng}, location = {Prague, Czech republic}, isbn = {8086241262}, pages = {87-90}, publisher = {Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic}, title = {Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides}, year = {2005} }
TY - JOUR ID - 701551 AU - Šulák, Ondřej - Spanilá, Miroslava - Gajdošová, Dagmar - Pazourek, Jiří - Havel, Josef PY - 2005 TI - Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides PB - Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic CY - Prague, Czech republic SN - 8086241262 KW - Capillary electrophoresis KW - Coating KW - Electroosmotic flow KW - Polyethyleneimine KW - 3glycidoxypropyltrimethoxysilane KW - 3aminopropyltriethoxysilane KW - Linear polyacrylamide N2 - Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system. ER -
ŠULÁK, Ondřej, Miroslava SPANILÁ, Dagmar GAJDOŠOVÁ, Jiří PAZOUREK and Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In \textit{Collection Symposium Series, Biologically Active Peptides}. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p.~87-90. ISBN~8086241262.
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