Diagnosis of tularemia using piezoelectric biosensor technology

J 2007

Diagnosis of tularemia using piezoelectric biosensor technology

POHANKA, Miroslav, Oto PAVLIŠ a Petr SKLÁDAL

Základní údaje

Originální název

Diagnosis of tularemia using piezoelectric biosensor technology

Název česky

Diagnostika tularemie použitím technologie piezoelektrického biosensoru

Autoři

POHANKA, Miroslav (203 Česká republika), Oto PAVLIŠ (203 Česká republika) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

Talanta, Amsterdam, Elsevier Science, 2007, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.374

Kód RIV

RIV/00216224:14310/07:00021740

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000244369600070

Klíčová slova anglicky

Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor

Štítky

bioterrorism, Francisella tularensis, immunosensor, Piezoelectric biosensor, rapid detection, Tularemia

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 7. 2007 10:40, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2.4% for IMS, respectively. The developed method allows to indicate the presence of anti-tularemic antibodies shortly (1 to 3 days) after infection, one analysis is completed in 10 min.

Česky

Detekce tularemie pomoci imunosensoru.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

POHANKA, Miroslav, Oto PAVLIŠ a Petr SKLÁDAL. Diagnosis of tularemia using piezoelectric biosensor technology. Talanta. Amsterdam: Elsevier Science, 2007, roč. 71, č. 1, s. 981-985. ISSN 0039-9140.

@article{701749,
   author = {Pohanka, Miroslav and Pavliš, Oto and Skládal, Petr},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Diagnosis of tularemia using piezoelectric biosensor technology},
   url = {http://dx.doi.org/10.1016/j.talanta.2006.05.074},
   volume = {71},
   year = {2007}
}

TY  - JOUR
ID  - 701749
AU  - Pohanka, Miroslav - Pavliš, Oto - Skládal, Petr
PY  - 2007
TI  - Diagnosis of tularemia using piezoelectric biosensor technology
JF  - Talanta
VL  - 71
IS  - 1
SP  - 981-985
EP  - 981-985
PB  - Elsevier Science
SN  - 00399140
KW  - Piezoelectric biosensor
KW  - Francisella tularensis
KW  - Bioterrorism
KW  - Tularemia
KW  - Rapid detection
KW  - Immunosensor
UR  - http://dx.doi.org/10.1016/j.talanta.2006.05.074
N2  - A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2.4% for IMS, respectively. The developed method allows to indicate the presence of anti-tularemic antibodies shortly (1 to 3 days) after infection, one analysis is completed in 10 min.
ER  -

POHANKA, Miroslav, Oto PAVLIŠ a Petr SKLÁDAL. Diagnosis of tularemia using piezoelectric biosensor technology. \textit{Talanta}. Amsterdam: Elsevier Science, 2007, roč.~71, č.~1, s.~981-985. ISSN~0039-9140.

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