Diagnosis of tularemia using piezoelectric biosensor technology

J 2007

Diagnosis of tularemia using piezoelectric biosensor technology

POHANKA, Miroslav, Oto PAVLIŠ and Petr SKLÁDAL

Basic information

Original name

Diagnosis of tularemia using piezoelectric biosensor technology

Name in Czech

Diagnostika tularemie použitím technologie piezoelektrického biosensoru

Authors

POHANKA, Miroslav (203 Czech Republic), Oto PAVLIŠ (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Talanta, Amsterdam, Elsevier Science, 2007, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.374

RIV identification code

RIV/00216224:14310/07:00021740

Organization unit

Faculty of Science

UT WoS

000244369600070

Keywords in English

Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor

Abstract

ORIG CZ

V originále

A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2.4% for IMS, respectively. The developed method allows to indicate the presence of anti-tularemic antibodies shortly (1 to 3 days) after infection, one analysis is completed in 10 min.

In Czech

Detekce tularemie pomoci imunosensoru.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

POHANKA, Miroslav, Oto PAVLIŠ and Petr SKLÁDAL. Diagnosis of tularemia using piezoelectric biosensor technology. Talanta. Amsterdam: Elsevier Science, 2007, vol. 71, No 1, p. 981-985. ISSN 0039-9140.

@article{701749,
   author = {Pohanka, Miroslav and Pavliš, Oto and Skládal, Petr},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Diagnosis of tularemia using piezoelectric biosensor technology},
   url = {http://dx.doi.org/10.1016/j.talanta.2006.05.074},
   volume = {71},
   year = {2007}
}

TY  - JOUR
ID  - 701749
AU  - Pohanka, Miroslav - Pavliš, Oto - Skládal, Petr
PY  - 2007
TI  - Diagnosis of tularemia using piezoelectric biosensor technology
JF  - Talanta
VL  - 71
IS  - 1
SP  - 981-985
EP  - 981-985
PB  - Elsevier Science
SN  - 00399140
KW  - Piezoelectric biosensor
KW  - Francisella tularensis
KW  - Bioterrorism
KW  - Tularemia
KW  - Rapid detection
KW  - Immunosensor
UR  - http://dx.doi.org/10.1016/j.talanta.2006.05.074
N2  - A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2.4% for IMS, respectively. The developed method allows to indicate the presence of anti-tularemic antibodies shortly (1 to 3 days) after infection, one analysis is completed in 10 min.
ER  -

POHANKA, Miroslav, Oto PAVLIŠ and Petr SKLÁDAL. Diagnosis of tularemia using piezoelectric biosensor technology. \textit{Talanta}. Amsterdam: Elsevier Science, 2007, vol.~71, No~1, p.~981-985. ISSN~0039-9140.

Detailed Information on Publication Record