Isolation and Expansion of Allogeneic Myeloma-Specific
Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

J 2006

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK et. al.

Základní údaje

Originální název

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

Název česky

Izolace a expanze alogenních myelom-specifických a interferon gama-pozitivních T lymfocytů pro adoptivní imunoterapii

Autoři

OČADLÍKOVÁ, Darina (203 Česká republika, garant), Lenka ZAHRADOVÁ (203 Česká republika), Lucie KOVÁŘOVÁ (203 Česká republika), Miroslav PENKA (203 Česká republika), Jan ŽALOUDÍK (203 Česká republika), Roman HÁJEK (203 Česká republika) a Jaroslav MICHÁLEK (203 Česká republika)

Vydání

Medical Oncology, Totowa, NJ, USA, Humana Press, 2006, 1559-131X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.256

Kód RIV

RIV/00216224:14110/06:00018909

Organizační jednotka

Lékařská fakulta

UT WoS

000242136000009

Klíčová slova anglicky

Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE

Štítky

CFSE, Immunotherapy, interferon-gamma, multiple myeloma, T cell

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 6. 2009 16:51, Mgr. Darina Očadlíková, Ph.D.

Anotace

ORIG CZ

V originále

Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.

Česky

Izolace a expanze alogenních myelom-specifických interferon-gama produkujících T lymfocytů pro buněčnou imunoterapii u mnohočetného myelomu.

Návaznosti

NC7475, projekt VaV

Název: Hodnocení buněčné imunity a molekulární analýza myelom-specifických T lymfocytů u pacientů s mnohočetným myelomem

Citovat

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK a Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. Medical Oncology. Totowa, NJ, USA: Humana Press, 2006, roč. 23/2006, č. 3, s. 377-384. ISSN 1559-131X.

@article{702247,
   author = {Očadlíková, Darina and Zahradová, Lenka and Kovářová, Lucie and Penka, Miroslav and Žaloudík, Jan and Hájek, Roman and Michálek, Jaroslav},
   article_location = {Totowa, NJ, USA},
   article_number = {3},
   keywords = {Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE},
   language = {eng},
   issn = {1559-131X},
   journal = {Medical Oncology},
   title = {Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy},
   volume = {23/2006},
   year = {2006}
}

TY  - JOUR
ID  - 702247
AU  - Očadlíková, Darina - Zahradová, Lenka - Kovářová, Lucie - Penka, Miroslav - Žaloudík, Jan - Hájek, Roman - Michálek, Jaroslav
PY  - 2006
TI  - Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
JF  - Medical Oncology
VL  - 23/2006
IS  - 3
SP  - 377-384
EP  - 377-384
PB  - Humana Press
SN  - 1559131X
KW  - Multiple myeloma
KW  - immunotherapy
KW  - interferon-gamma
KW  - T cell;CFSE
N2  - Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
ER  -

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK a Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. \textit{Medical Oncology}. Totowa, NJ, USA: Humana Press, 2006, roč.~23/2006, č.~3, s.~377-384. ISSN~1559-131X.

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