SLUNSKÁ, Zdenka, Iva SLANINOVÁ, Jiří ŠINKORA, Marcela VLKOVÁ and Eva TÁBORSKÁ. The cytotoxicity of minority benzo[c]phenanthridine alkaloids. In Books of abstracts of CHEMISTRY, PHARMACOLOGY AND BIOSYNTHESIS OF ALKALOIDS. Turkey: The Phytochemical Society of Europe, 2006. p. 90-90.
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Basic information
Original name The cytotoxicity of minority benzo[c]phenanthridine alkaloids
Name in Czech Cytotoxicita minoritních benzo[c]phenanthridinových alkaloidů
Authors SLUNSKÁ, Zdenka, Iva SLANINOVÁ, Jiří ŠINKORA, Marcela VLKOVÁ and Eva TÁBORSKÁ.
Edition Turkey, Books of abstracts of CHEMISTRY, PHARMACOLOGY AND BIOSYNTHESIS OF ALKALOIDS, p. 90-90, 1 pp. 2006.
Publisher The Phytochemical Society of Europe
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Turkey
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Medicine
Keywords in English benzo[c]phenanthridine alkaloids
Tags Benzo[c]phenanthridine Alkaloids
Tags International impact, Reviewed
Changed by Changed by: Mgr. Zdenka Slunská, Ph.D., učo 152406. Changed: 29/6/2007 12:19.
Abstract
In this study we determined cytotoxicity of sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL) and macarpine (MA), minor occuring quaternary benzo[c]phenanthridine alkaloids (QBA), in comparison with the two best known QBA sanguinarine (SA) and chelerythrine (CHE). QBA are a small group of isoquinoline alkaloids that are distributed in a plant species of Papaveraceae, Fumariaceae and Rutaceae families. SA and CHE exhibit wide spectrum of biological activities, while the information about the biological effects of minority QBA is rare. Our attention was pointed to study antiproliferative and pro-apoptotic activities of QBA and their influence on cytoskeleton. The antiproliferative activity was assessed by MTT metabolic assay. One normal human (LEP), one hamster (V-79) and three human tumour cell lines (HeLa; HL-60; A-2780) were used as a model objects. Our results showed that IC50 values after 72 hours treatment of alkaloids were comparable with IC50 of routinely used cytostatics (i.e.: 0,01-1 microgram/ml). HL-60 was the most sensitive cell line while HeLa cells appeared to be the most resistant. For apoptosis detection morphology of nuclei of alkaloid treated HL-60 cells after DAPI staining was studied and flow cytometric analysis using Annexin V-FITC and propidium iodide was applied. All tested alkaloids appeared to be inductors of apoptosis at the concentration 1 microgram/ml. Based on our observations we concluded that alkaloids tested in this study show strong antiproliferative, proapoptotic effect and effect on cytoskeleton.
Abstract (in Czech)
Naše pozornost byla věnována studiu antiproliferačního a proapoptotického účinku benzo[c]phenanthridinových alkaloidů a také jejich vlivu na cytoskelet. Antiproliferační aktivita byla stanovena s využitím MTT testu metabolické aktivity a jako modelové objekty sloužily dvě nenádorové a tři nádorové buněčné linie. Naše výsledky ukazují, že naměřené hodnoty IC50 jsou srovnatelné s hodnotami IC50 běžně používaných cytostatik. Na působení alkaloidů byla nejcitlivější buněčná linie HL-60 (buňky lidské promyelocytární leukemie). Indukce apoptotické buněčné smrti byla studována pomocí flow cytometrické analýzy po barvení Annexinem V-FITC a propidium jodidem. Všechny testované alkaloidy jsou induktory apoptózy v koncentraci 1 mikrogram/ml.
Links
GA525/04/0017, research and development projectName: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky
Investor: Czech Science Foundation, Standard Projects
GD301/03/H005, research and development projectName: Molekulární a buněčná biologie v biomedicíně
Investor: Czech Science Foundation, Doctor grants
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