| ONDROUŠKOVÁ, Eva, Karolína POVOLNÁ, Petr VÁŇA, Petr BENEŠ, Hana KONEČNÁ, Zbyněk ZDRÁHAL a Jan ŠMARDA. A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts. Leukemia Research. Elsevier, 2007, roč. 31, č. 2, s. 221-229. ISSN 0145-2126. |
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@article{705140,
author = {Ondroušková, Eva and Povolná, Karolína and Váňa, Petr and Beneš, Petr and Konečná, Hana and Zdráhal, Zbyněk and Šmarda, Jan},
article_number = {2},
keywords = {v-myb; monoblast; macrophage; differentiation; proteome; two-dimensional gel electrophoresis; trichostatin A; TPA; mass spectrometry},
language = {eng},
issn = {0145-2126},
journal = {Leukemia Research},
title = {A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts},
volume = {31},
year = {2007}
}
TY - JOUR
ID - 705140
AU - Ondroušková, Eva - Povolná, Karolína - Váňa, Petr - Beneš, Petr - Konečná, Hana - Zdráhal, Zbyněk - Šmarda, Jan
PY - 2007
TI - A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts
JF - Leukemia Research
VL - 31
IS - 2
SP - 221-229
EP - 221-229
PB - Elsevier
SN - 01452126
KW - v-myb
KW - monoblast
KW - macrophage
KW - differentiation
KW - proteome
KW - two-dimensional gel electrophoresis
KW - trichostatin A
KW - TPA
KW - mass spectrometry
N2 - v-myb-oncogene of avian myeloblastosis virus transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation.
ER -
ONDROUŠKOVÁ, Eva, Karolína POVOLNÁ, Petr VÁŇA, Petr BENEŠ, Hana KONEČNÁ, Zbyněk ZDRÁHAL a Jan ŠMARDA. A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts. \textit{Leukemia Research}. Elsevier, 2007, roč.~31, č.~2, s.~221-229. ISSN~0145-2126.
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