Detection of chromosomal aberrations by FISH, CGH and SKY in
cultured and noncultured tumours in children during 2003 and
2005

D 2006

Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ et. al.

Basic information

Original name

Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005

Name in Czech

Detekce chromozomových aberací u kultivovaných a nekultivovaných dětských solidních nádorů pomocí techniky FISH, CGH a SKY v průběhu let 2003 až 2005

Authors

OLTOVÁ, Alexandra (203 Czech Republic), Petra ŠMUHAŘOVÁ (203 Czech Republic), Petr KUGLÍK (203 Czech Republic, guarantor), Dita ŽEŽULKOVÁ (203 Czech Republic), Vladimíra VRANOVÁ (703 Slovakia), Peter MÚDRY (203 Czech Republic), Věra BAIČIOVÁ (203 Czech Republic) and Jaroslav ŠTĚRBA (203 Czech Republic)

Edition

Amsterdam, Europen Journal of Human Genetics, p. 226-226, 2006

Publisher

ESHG

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00017836

Organization unit

Faculty of Science

ISBN

80-7232-264-8

Keywords in English

Chromosomal aberrations; solid tumours in children; FISH; CGH; SKY

Abstract

ORIG CZ

V originále

Pediatric tumors are the second main cause of childrens death in developed countries. Contrary to leukemias chromosomal alterations associated with malignant transformations are less well characterized for pediatric solid tumors. We present results of cytogenetic examinations of 262 pediatric solid tumors. Some of them were examined using by interphase fluorescent in situ hybridization (I-FISH), especially tumors where deletions or amplifications of specific genes and/or presence of fusion genes are known and could be used as diagnostic tool. Proven prognostic significance of the n-myc amplification, del 1p36, gain 17q, abnormalities on chromosome 11 are well known for children with neuroblastoma. For medulloblastomas i(17p), c-myc and i(12p) in germinal tumors are the other candidates. Vast majority of tumor samples were cultured and other possible chromosomal abnormalities were detected using FISH, spectral karyotyping (SKY) and comparative genomic hybridization (CGH). During last few months we have applied also high-resolution comparative genomic hybridization (HR-CGH). Cultivation of solid tumor tissue is not always successful, possible explanation could be generally low proliferation activity of tumor cells in vitro. Here we present our cytogenetic results together with clinical characteristics.

In Czech

Práce se zabývá detekcí chromozomových aberací ve vzorcích kultivované a nekultivované tkáně solidních dětských nádorů pomocí techniky FISH, CGH a spektrálního karyotypování (SKY) Jsou zhodnoceny cytogenetické nálezy u pacientů léčených na KDO FN Brno v letech 2003-2005.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ, Peter MÚDRY, Věra BAIČIOVÁ and Jaroslav ŠTĚRBA. Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005. In Europen Journal of Human Genetics. Amsterdam: ESHG, 2006, p. 226. ISBN 80-7232-264-8.

@inproceedings{705152,
   author = {Oltová, Alexandra and Šmuhařová, Petra and Kuglík, Petr and Žežulková, Dita and Vranová, Vladimíra and Múdry, Peter and Baičiová, Věra and Štěrba, Jaroslav},
   address = {Amsterdam},
   booktitle = {Europen Journal of Human Genetics},
   keywords = {Chromosomal aberrations; solid tumours in children; FISH; CGH; SKY},
   language = {eng},
   location = {Amsterdam},
   isbn = {80-7232-264-8},
   pages = {226-226},
   publisher = {ESHG},
   title = {Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005},
   year = {2006}
}

TY  - JOUR
ID  - 705152
AU  - Oltová, Alexandra - Šmuhařová, Petra - Kuglík, Petr - Žežulková, Dita - Vranová, Vladimíra - Múdry, Peter - Baičiová, Věra - Štěrba, Jaroslav
PY  - 2006
TI  - Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005
PB  - ESHG
CY  - Amsterdam
SN  - 8072322648
KW  - Chromosomal aberrations
KW  - solid tumours in children
KW  - FISH
KW  - CGH
KW  - SKY
N2  - Pediatric tumors are the second main cause of childrens death in developed countries. Contrary to leukemias chromosomal alterations associated with malignant transformations are less well characterized for pediatric solid tumors. We present results of cytogenetic examinations of 262 pediatric solid tumors. Some of them were examined using by interphase fluorescent in situ hybridization (I-FISH), especially tumors where deletions or amplifications of specific genes and/or presence of fusion genes are known and could be used as diagnostic tool. Proven prognostic significance of the n-myc amplification, del 1p36, gain 17q, abnormalities on chromosome 11 are well known for children with neuroblastoma. For medulloblastomas i(17p), c-myc and i(12p) in germinal tumors are the other candidates. Vast majority of tumor samples were cultured and other possible chromosomal abnormalities were detected using FISH, spectral karyotyping (SKY) and comparative genomic hybridization (CGH). During last few months we have applied also high-resolution comparative genomic hybridization (HR-CGH). Cultivation of solid tumor tissue is not always successful, possible explanation could be generally low proliferation activity of tumor cells in vitro. Here we present our cytogenetic results together with clinical characteristics.
ER  -

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ, Peter MÚDRY, Věra BAIČIOVÁ and Jaroslav ŠTĚRBA. Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005. In \textit{Europen Journal of Human Genetics}. Amsterdam: ESHG, 2006, p.~226. ISBN~80-7232-264-8.

Detailed Information on Publication Record