| RITTICH, Bohuslav, Jana KŘÍŽOVÁ, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria. In Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9. York, Velká Británie: Royal Society of Chemistry, 2006, s. P167, 1 s. |
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@inproceedings{706303,
author = {Rittich, Bohuslav and Křížová, Jana and Španová, Alena and Němcová, Petra and Horák, Daniel},
address = {York, Velká Británie},
booktitle = {Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9},
keywords = {Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility},
language = {eng},
location = {York, Velká Británie},
pages = {P167},
publisher = {Royal Society of Chemistry},
title = {Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria},
year = {2006}
}
TY - JOUR
ID - 706303
AU - Rittich, Bohuslav - Křížová, Jana - Španová, Alena - Němcová, Petra - Horák, Daniel
PY - 2006
TI - Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria
PB - Royal Society of Chemistry
CY - York, Velká Británie
KW - Magnetic particles
KW - HEMA-co-GMA
KW - Bifidobacterium
KW - RAPD
KW - rep-PCR
KW - reproducibility
N2 - DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method.
ER -
RITTICH, Bohuslav, Jana KŘÍŽOVÁ, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria. In \textit{Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9}. York, Velká Británie: Royal Society of Chemistry, 2006, s.~P167, 1 s.
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