Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria

RITTICH, Bohuslav, Jana KŘÍŽOVÁ, Alena ŠPANOVÁ, Petra NĚMCOVÁ and Daniel HORÁK. Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria. In Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9. York, Velká Británie: Royal Society of Chemistry, 2006, p. P167, 1 pp.

Basic information

• Original name: Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria
• Name in Czech: Vliv magnetických částic na reprodukovatelnost RAPD a rep-PCR fingrprintů
• Authors: RITTICH, Bohuslav (203 Czech Republic, guarantor), Jana KŘÍŽOVÁ (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic), Petra NĚMCOVÁ (203 Czech Republic) and Daniel HORÁK (203 Czech Republic).
• Edition: York, Velká Británie, Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9, p. P167, 1 pp. 2006.
• Publisher: Royal Society of Chemistry

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/06:00024563
• Organization unit: Faculty of Science
• Keywords in English: Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility
• Tags: Bifidobacterium, HEMA-co-GMA, magnetic particles, RAPD, rep-PCR, reproducibility

Abstract

DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method.

Abstract (in Czech)

DNA byla izolována z buněk bifidobakterií pomocí fenolové extrakce, vysolováním 6 M NaCl a adsorpcí na magnetické poly(2-hydroxyethyl methakrylát-co-glycidyl methakrylát) (HEMA-co-GMA) částice pokryté karboxylovými skupinami. Bylo zjištěno, že DNA eluovaná z magnetických částic za použití TE pufru může být použita při tvorbě fingrprintů. Reprodukovatelnost testovaných metod byla srovnatelná s fenolovou extrakcí.

Links

• GA203/05/2256, research and development project: Name: Magnetické hydrofilní polymerní mikročástice reagující na vnější stimuly pro lékařství a bioinženýrství

Investor: Czech Science Foundation, Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• 1G46045, research and development project: Name: Molekulárně biologická analýza tuzemského fondu produkčních kmenů baktérií mléčného kvašení.

Investor: Ministry of Agriculture of the CR, Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods