| ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK, Alois RYBNIKÁŘ, Igor ŠTYRIAK, V. JŮZOVÁ, L. ROHÁNKOVÁ, B. RUTTKAY-NEDECKÝ a P. KUČERA. Use of magnetic hydrophilic particles for DNA isolation from dairy products and unusual samples. In Ninth International symposium on hyphenated techniques in Chromatography and Hyphenated Chromatographic analyzers, HTC-9. York, Velká Británie: Royal Society of Chemistry, 2006, s. P196, 1 s. |
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@inproceedings{706308,
author = {Španová, Alena and Rittich, Bohuslav and Horák, Daniel and Rybnikář, Alois and Štyriak, Igor and Jůzová, V. and Rohánková, L. and RuttkayandNedecký, B. and Kučera, P.},
address = {York, Velká Británie},
booktitle = {Ninth International symposium on hyphenated techniques in Chromatography and Hyphenated Chromatographic analyzers, HTC-9},
keywords = {Magnetic particles; DNA; dairy products; Trychophyton cells; Lake Baikal sediments; GMO; PCR},
language = {eng},
location = {York, Velká Británie},
pages = {P196},
publisher = {Royal Society of Chemistry},
title = {Use of magnetic hydrophilic particles for DNA isolation from dairy products and unusual samples},
year = {2006}
}
TY - JOUR
ID - 706308
AU - Španová, Alena - Rittich, Bohuslav - Horák, Daniel - Rybnikář, Alois - Štyriak, Igor - Jůzová, V. - Rohánková, L. - Ruttkay-Nedecký, B. - Kučera, P.
PY - 2006
TI - Use of magnetic hydrophilic particles for DNA isolation from dairy products and unusual samples
PB - Royal Society of Chemistry
CY - York, Velká Británie
KW - Magnetic particles
KW - DNA
KW - dairy products
KW - Trychophyton cells
KW - Lake Baikal sediments
KW - GMO
KW - PCR
N2 - Magnetic hydrophilic methacrylate–based polymer microspheres containing carboxyl groups were used for isolation of bacterial DNA from differnt samples. DNA was adsorbed on particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations. The eluted DNA was used directly for PCR identification of Salmonella cells containing intracellular PCR inhibitors, Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products), Trichophyton cells in lyophilisates, bacterial cells in sediments from Lake Baikal, and GMO testing (DNA isolated from rose soya granulate).
ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK, Alois RYBNIKÁŘ, Igor ŠTYRIAK, V. JŮZOVÁ, L. ROHÁNKOVÁ, B. RUTTKAY-NEDECKÝ a P. KUČERA. Use of magnetic hydrophilic particles for DNA isolation from dairy products and unusual samples. In \textit{Ninth International symposium on hyphenated techniques in Chromatography and Hyphenated Chromatographic analyzers, HTC-9}. York, Velká Británie: Royal Society of Chemistry, 2006, s.~P196, 1 s.
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