Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761

ŠPANOVÁ, Alena, Jana KŘÍŽOVÁ, Radka BURDYCHOVÁ, Vladimír DRÁB and Bohuslav RITTICH. Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761. In 10th International symposium on the genetics of industrial microorganisms. Praha: Mikrobiologický ústav AV ČR, Praha, 2006, p. P046, 1 pp. ISBN 80-239-7323-1.

Basic information

• Original name: Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761
• Name in Czech: taxonomická analýza a reklasifikace Bifidobacterium adolescentis CCM 3761
• Authors: ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Jana KŘÍŽOVÁ (203 Czech Republic), Radka BURDYCHOVÁ (203 Czech Republic), Vladimír DRÁB (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic).
• Edition: Praha, 10th International symposium on the genetics of industrial microorganisms, p. P046, 1 pp. 2006.
• Publisher: Mikrobiologický ústav AV ČR, Praha

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/06:00031777
• Organization unit: Faculty of Science
• ISBN: 80-239-7323-1
• Keywords in English: Bifidobacterium; ARDRA; RAPD; rep-PCR; sequencing; species identification
• Tags: ARDRA, Bifidobacterium, RAPD, rep-PCR, Sequencing, species identification
• Tags: International impact

Abstract

DNA fingerprinting methods (ARDRA, RAPD, rep-PCR) have been applied for discrimination of Bifidobacterium species. Sixteen Bifidobacterium strains of eleven species which were used to evaluate the discrimination power of the above-mentioned methods were obtained from the Czech Collection of Microorganisms (CCM, Brno, Czech Republic), the American Type Culture Collection (ATCC, Manassas, USA), and the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). In characterisation of the studied strains it was found that the results obtained for the strain B. adolescentis CCM 3761 did not correspond with its taxonomic position and the strain should be reclassified as B. pseudocatenulatum. The amplicons of the 16S rDNA region of B. adolescentis CCM 3761 and B. catenulatum CCM 7094, B. pseudocatenulatum DSM 16992T, and B. adolescentis CCM 4987T were used for sequencing of DNA and for confirmation of species identification.

Abstract (in Czech)

Typizační metody (ARDRA, RAPD, rep-PCR) byly použity pro rozlišení bifidobakterií. K ověření rozlišovací schopnosti výše uvedených metod byly použity sbírkové kmeny (CCM, ATCC, DSZM). Bylo zjištěno, že kmen Bifidobacterium adolescentis CCM 3761 neodpovídá jeho taxonomické pozici a kmen byl reklasifikován jako B. pseudocatenulatum. Amplikony z úseku 16S rDNA kmenů B. adolescentis CCM 3761,B. catenulatum CCM 7094, B. pseudocatenulatum DSM 16992T a B. adolescentis CCM 4987T byly použity k sekvencování DNA a potvrzení druhové identifikace.

Links

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• QF3169, research and development project: Name: Využití postupů na bázi molekulární genetiky při získávání nových kmenů baktérií s probiotickými vlastnostmi s cílem rozšíření specifických genetických zdrojů pro výrobu funkčních potravin

Investor: Ministry of Agriculture of the CR, The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods