| RITTICH, Bohuslav, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Use of an aqueous two-phase system and solid magnetic hydrophilic particles for isolationof PCR-ready DNA from dairy products. In 26th International symposium on Separation of Proteins, Peptides and Polynucleotides. Innsbruck: Dechema, 2006, s. 76-76. |
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@inproceedings{707285,
author = {Rittich, Bohuslav and Španová, Alena and Němcová, Petra and Horák, Daniel},
address = {Innsbruck},
booktitle = {26th International symposium on Separation of Proteins, Peptides and Polynucleotides},
keywords = {Bifidobacterium; Dairy products; Identification; PCR; P(HEMA-co-EDMA) magnetic particles; Two-phase system},
language = {eng},
location = {Innsbruck},
pages = {76-76},
publisher = {Dechema},
title = {Use of an aqueous two-phase system and solid magnetic hydrophilic particles for isolationof PCR-ready DNA from dairy products},
year = {2006}
}
TY - JOUR
ID - 707285
AU - Rittich, Bohuslav - Španová, Alena - Němcová, Petra - Horák, Daniel
PY - 2006
TI - Use of an aqueous two-phase system and solid magnetic hydrophilic particles for isolationof PCR-ready DNA from dairy products
PB - Dechema
CY - Innsbruck
KW - Bifidobacterium
KW - Dairy products
KW - Identification
KW - PCR
KW - P(HEMA-co-EDMA) magnetic particles
KW - Two-phase system
N2 - The identification of bacteria in real samples implies several steps: preparation of PCR-ready DNA and identification of bacterial strains by PCR. In some samples of dairy products the sensitivity of PCR was decreased due to the presence of PCR inhibitors. In these cases PCR-ready DNAs were isolated using an aqueous two-phase system, composed of 16 % (w/v) PEG 6000, NaCl and solid magnetic hydrophilic (P(HEMA-co-EDMA)) microspheres containing carboxyl groups. The method developed was applied for isolation of PCR-ready DNA from Bifidobacterium cells in some dairy products (different yoghurt samples). PCR was performed using PbiF1 and PbiR2 primers which enable amplification of a 914 bp long DNA fragment specific to the Bifidobacterium genus (Roy and Sirois, 2000). PCR products were detected using electrophoresis in 1.5 % agarose gel in TBE buffer.
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Use of an aqueous two-phase system and solid magnetic hydrophilic particles for isolationof PCR-ready DNA from dairy products. In \textit{26th International symposium on Separation of Proteins, Peptides and Polynucleotides}. Innsbruck: Dechema, 2006, s.~76-76.
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