Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase
defines an essential function for the Sgs1-Top3 complex in the
absence of SRS2 or TOP1.

J 2000

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ, Christian BENDIXEN et. al.

Základní údaje

Originální název

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

Název česky

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

Autoři

DUNO, Morten (208 Dánsko), Bo THOMSEN (208 Dánsko), Ole WESTERGAARD (208 Dánsko), Lumír KREJČÍ (203 Česká republika, garant) a Christian BENDIXEN (208 Dánsko)

Vydání

Mol Gen Genet, 2000, 1617-4615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Recombination; repair; Sgs1;Top3

Štítky

recombination, repair, Sgs1, Top3

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 5. 2009 23:04, doc. Mgr. Lumír Krejčí, Ph.D.

Anotace

ORIG CZ

V originále

The Saccharomyces cerevisiae gene SGS1 encodes a DNA helicase that shows homology to the Escherichia coli protein RecQ and the products of the BLM and WRN genes in humans, which are defective in Bloom's and Werner's syndrome, respectively. Recently, it has been proposed that this helicase is involved in maintaining the integrity of the rDNA and that loss of Sgs1 function leads to accelerated aging. Sgs1 has been isolated on the basis of its genetic interaction with both topoisomerase I and topoisomerase III, as well as in a two-hybrid screen for proteins that interact with the C-terminal portion of topoisomerase II. We have defined the minimal structural elements of Sgs1 required for its interactions with the three topoisomerases, and demonstrate that the complex phenotypes associated with sgs1 mutants are a consequence of a dysfunctional Sgs1-Top3 complex. We also report that the synthetic relationship between mutations in SGS1 and SRS2, which encodes another helicase implicated in recombinational repair, likewise result from a dysfunctional Sgs1-Top3 interaction. Our findings indicate that Sgs1 may act on different DNA structures depending on the activity of topoisomerase I, Srs2 and topoisomerase III.

Česky

Genetická charakterizace SGS1 a TOP1 vztahů

Citovat

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ a Christian BENDIXEN. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. Mol Gen Genet. 2000, roč. 264, 1-2, s. 89-97, 8 s. ISSN 1617-4615.

@article{708570,
   author = {Duno, Morten and Thomsen, Bo and Westergaard, Ole and Krejčí, Lumír and Bendixen, Christian},
   article_number = {1-2},
   keywords = {Recombination; repair; Sgs1;Top3},
   language = {eng},
   issn = {1617-4615},
   journal = {Mol Gen Genet},
   title = {Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.},
   volume = {264},
   year = {2000}
}

TY  - JOUR
ID  - 708570
AU  - Duno, Morten - Thomsen, Bo - Westergaard, Ole - Krejčí, Lumír - Bendixen, Christian
PY  - 2000
TI  - Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.
JF  - Mol Gen Genet
VL  - 264
IS  - 1-2
SP  - 89-97
EP  - 89-97
SN  - 16174615
KW  - Recombination
KW  - repair
KW  - Sgs1;Top3
N2  - The Saccharomyces cerevisiae gene SGS1 encodes a DNA helicase that shows homology to the Escherichia coli protein RecQ and the products of the BLM and WRN genes in humans, which are defective in Bloom's and Werner's syndrome, respectively. Recently, it has been proposed that this helicase is involved in maintaining the integrity of the rDNA and that loss of Sgs1 function leads to accelerated aging. Sgs1 has been isolated on the basis of its genetic interaction with both topoisomerase I and topoisomerase III, as well as in a two-hybrid screen for proteins that interact with the C-terminal portion of topoisomerase II. We have defined the minimal structural elements of Sgs1 required for its interactions with the three topoisomerases, and demonstrate that the complex phenotypes associated with sgs1 mutants are a consequence of a dysfunctional Sgs1-Top3 complex. We also report that the synthetic relationship between mutations in SGS1 and SRS2, which encodes another helicase implicated in recombinational repair, likewise result from a dysfunctional Sgs1-Top3 interaction. Our findings indicate that Sgs1 may act on different DNA structures depending on the activity of topoisomerase I, Srs2 and topoisomerase III.
ER  -

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ a Christian BENDIXEN. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. \textit{Mol Gen Genet}. 2000, roč.~264, 1-2, s.~89-97, 8 s. ISSN~1617-4615.

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