Monitoring of cell adhesion and cytotoxicity effect of
derivative vitamine E using a piezoelectric biosensor

D 2006

Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor

FOHLEROVÁ, Zdenka, Petr SKLÁDAL a Jaroslav TURÁNEK

Základní údaje

Originální název

Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor

Název česky

Sledování buněčné adheze a cytotoxických efektů derivátů vitaminu E použitím piezoelektrického biosensoru

Autoři

FOHLEROVÁ, Zdenka, Petr SKLÁDAL a Jaroslav TURÁNEK

Vydání

Amsterdam, The 9th World Congress on Biosensors - Delegate Manual, s. 11-11, 2006

Nakladatel

Elsevier

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Piezoelectric biosensor; Resistance; Cytotoxicity assay

Štítky

Cytotoxicity assay, Piezoelectric biosensor, resistance

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 7. 2008 10:14, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing living rat epithelial cells WB F344 and lung melanoma cells B16F10 as biorecognition elements. The resonant frequency delta-f and resistance delta-R changes were measured during adhesion of the cells on the gold surfaces modified by the layers of either hydrophobic polystyrene or extracellular matrix proteins as fibronectin, laminin and vitronectin. The piezoelectric biosensor was furthermore utlized for monitoring of apoptosis initiated by the addition of vitamin E derivatives as a-tocopheryl amidomalate (a-TAM). Next, the impedance scans were used to followed the conductivity of gap junctions of the WB F344 cell line before and after the cytotoxic effects of several anticancer drugs. The 10 MHz quartz crystal with the gold electrodes on the both sides was placed at the bottom of the thermostated measurement chamber. The cells adhered on the gold surface were identified by staining with MitoTracker Red fluorescent dye. The concentration of a-TAM added to the cell medium was 300 uM. The process on the sensor was simultaneously observed and compared on microplates. The initial cell attachment and spreading induced a decrease of frequency and increase of resistance. The steady-state of both shifts was achieved after a few hours. The presence of cells on the surface was confirmed by fluorescent microscopy. The apoptosis was morphologically characterized by severe alterations in the cell shape like shrinkage and disintegration of cell-cell contacts and therefore it was sensitively measured by QCM. Consequently, the decreased frequency and increased resistance values were observed after the addition of a-TAM. The information from the simultaneous on-line monitoring of both f and R changes revealed viscoelastic properties of the cell monolayer on the sensing surface. As the attached cells formed an insulating layer, the observation of the conductivity of the gap junction after the drug treatment was shown as the increase of the conductivity. The results indicate that the piezoelectric biosensor is suitable for real-time studies of cell adhesion and for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.

Česky

Piezoelektrický sensor byl modifikován různými látkami usnadňujícími adhezi savčích buněk. Jako parametry adheze byly kontinuálně sledovány resonanční frekvence a resistance sensoru. Po adhezi buněk byly takto získané biosensory využity pro sledování cytotoxických efektů různých látek, například derivátů vitaminu E.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

FOHLEROVÁ, Zdenka, Petr SKLÁDAL a Jaroslav TURÁNEK. Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor. In The 9th World Congress on Biosensors - Delegate Manual. Amsterdam: Elsevier, 2006, s. 11.

@inproceedings{708776,
   author = {Fohlerová, Zdenka and Skládal, Petr and Turánek, Jaroslav},
   address = {Amsterdam},
   booktitle = {The 9th World Congress on Biosensors - Delegate Manual},
   keywords = {Piezoelectric biosensor; Resistance; Cytotoxicity assay},
   language = {eng},
   location = {Amsterdam},
   pages = {11-11},
   publisher = {Elsevier},
   title = {Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor},
   year = {2006}
}

TY  - JOUR
ID  - 708776
AU  - Fohlerová, Zdenka - Skládal, Petr - Turánek, Jaroslav
PY  - 2006
TI  - Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor
PB  - Elsevier
CY  - Amsterdam
KW  - Piezoelectric biosensor
KW  - Resistance
KW  - Cytotoxicity assay
N2  - The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing living rat epithelial cells WB F344 and lung melanoma cells B16F10 as biorecognition elements. The resonant frequency delta-f and resistance delta-R changes were measured during adhesion of the cells on the gold surfaces modified by the layers of either hydrophobic polystyrene or extracellular matrix proteins as fibronectin, laminin and vitronectin. The piezoelectric biosensor was furthermore utlized for monitoring of apoptosis initiated by the addition of vitamin E derivatives as a-tocopheryl amidomalate (a-TAM). Next, the impedance scans were used to followed the conductivity of gap junctions of the WB F344 cell line before and after the cytotoxic effects of several anticancer drugs. The 10 MHz quartz crystal with the gold electrodes on the both sides was placed at the bottom of the thermostated measurement chamber. The cells adhered on the gold surface were identified by staining with MitoTracker Red fluorescent dye. The concentration of a-TAM added to the cell medium was 300 uM. The process on the sensor was simultaneously observed and compared on microplates. The initial cell attachment and spreading induced a decrease of frequency and increase of resistance. The steady-state of both shifts was achieved after a few hours. The presence of cells on the surface was confirmed by fluorescent microscopy. The apoptosis was morphologically characterized by severe alterations in the cell shape like shrinkage and disintegration of cell-cell contacts and therefore it was sensitively measured by QCM. Consequently, the decreased frequency and increased resistance values were observed after the addition of a-TAM. The information from the simultaneous on-line monitoring of both f and R changes revealed viscoelastic properties of the cell monolayer on the sensing surface. As the attached cells formed an insulating layer, the observation of the conductivity of the gap junction after the drug treatment was shown as the increase of the conductivity. The results indicate that the piezoelectric biosensor is suitable for real-time studies of cell adhesion and for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.
ER  -

FOHLEROVÁ, Zdenka, Petr SKLÁDAL a Jaroslav TURÁNEK. Monitoring of cell adhesion and cytotoxicity effect of derivative vitamine E using a piezoelectric biosensor. In \textit{The 9th World Congress on Biosensors - Delegate Manual}. Amsterdam: Elsevier, 2006, s.~11.

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