2006
A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS
BUDILOVÁ, Jana, Jiří PAZOUREK, Pavel KRÁSENSKÝ a Jan POSPÍCHALZákladní údaje
Originální název
A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS
Název česky
A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS
Autoři
BUDILOVÁ, Jana (203 Česká republika), Jiří PAZOUREK (203 Česká republika, garant), Pavel KRÁSENSKÝ (203 Česká republika) a Jan POSPÍCHAL (203 Česká republika)
Vydání
Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2006, 1615-9306
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.535
Kód RIV
RIV/00216224:14310/06:00016304
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000239574100011
Klíčová slova anglicky
capillary isoelectric focusing;neutralization reaction boundary;proteins;fractionation;injected large volume sample
Štítky
Příznaky
Recenzováno
Změněno: 26. 1. 2007 12:55, doc. RNDr. Bc. Jiří Pazourek, Ph.D.
V originále
Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). Concentration in fractions was increased up to 30 times for myoglobin and 10 times for insulin, respectively.
Česky
Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). Concentration in fractions was increased up to 30 times for myoglobin and 10 times for insulin, respectively.
Návaznosti
| GA305/03/1100, projekt VaV |
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