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@inproceedings{709266, author = {Horák, Daniel and Rittich, Bohuslav and Španová, Alena}, address = {Krems}, booktitle = {6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers}, keywords = {Magnetic particles; praparation; DNA separation; Lactobacillus; Bifidobacterium; PCR}, language = {eng}, location = {Krems}, pages = {17-17}, publisher = {University of applied Sciences Krems, Austria}, title = {Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in Dairy products}, year = {2006} }
TY - JOUR ID - 709266 AU - Horák, Daniel - Rittich, Bohuslav - Španová, Alena PY - 2006 TI - Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in Dairy products PB - University of applied Sciences Krems, Austria CY - Krems KW - Magnetic particles KW - praparation KW - DNA separation KW - Lactobacillus KW - Bifidobacterium KW - PCR N2 - Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. ER -
HORÁK, Daniel, Bohuslav RITTICH a Alena ŠPANOVÁ. Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in Dairy products. In \textit{6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers}. Krems: University of applied Sciences Krems, Austria, 2006, s.~17-17.
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