Evaluation of xenoestrogenic potential of propylparaben in zebrafish (Danio rerio)

MIKULA, P., R. DOBŠÍKOVÁ, Z. SVOBODOVÁ and Jiří JARKOVSKÝ. Evaluation of xenoestrogenic potential of propylparaben in zebrafish (Danio rerio). Neuroendocrinology Letters. 2006, vol. 27, Suppl.2, p. 101-107, 4 pp. ISSN 0172-780X.

Basic information

• Original name: Evaluation of xenoestrogenic potential of propylparaben in zebrafish (Danio rerio)
• Name in Czech: Evaluation of xenoestrogenic potential of propylparaben in zebrafish (Danio rerio)
• Authors: MIKULA, P. (203 Czech Republic), R. DOBŠÍKOVÁ (203 Czech Republic), Z. SVOBODOVÁ (203 Czech Republic) and Jiří JARKOVSKÝ (203 Czech Republic, guarantor).
• Edition: Neuroendocrinology Letters, 2006, 0172-780X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10511 Environmental sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.924
• RIV identification code: RIV/00216224:14310/06:00018449
• Organization unit: Faculty of Science
• UT WoS: 000245442600025
• Keywords in English: endocrine disruptors; parabens; preservatives; vitellogenin; xenoestrogens
• Tags: endocrine disruptors, parabens, preservatives, vitellogenin, xenoestrogens

Abstract

OBJECTIVES: Xenoestrogenic potential of propylparaben (PP), one of the most commonly used preservatives in drugs, cosmetics and food, was investigated in vivo using zebrafish (Danio rerio). METHODS: Juvenile zebrafish (20 days post hatching) were exposed to three different concentrations of propylparaben (PP) dissolved in ethanol and added into the water. After 20 days of exposure the fish were euthanized and vitellogenin concentrations in their whole body homogenates were measured by the method of direct sandwich ELISA. Simultaneously, vitellogenin concentrations in either fish from the control group (exposed to solvent without the substance tested) and in fish from the positive control group (exposed to natural estrogen 17B-estradiol) were measured. RESULTS: Vitellogenin concentration in whole body homogenates of control fish was 400 (396–540) ng.ml–1 (geometric mean (95% CI)). Zebrafish exposure to propylparaben at the concentrations of 0.1; 0.4 and 0.9 mg.l–1 elicited statistically significant decline (P<0.001) of vitellogenin production, i.e. geometric means of vitellogenin concentrations in whole body homogenates were 240 (186–311); 218 (175–270) and 270 (234–311) ng.ml–1, respectively. Conversely, the geometric mean of vitellogenin concentration in whole body homogenates of zebrafish exposed to 100 ng.l–1 of 17B-estradiol (positive control) was significantly higher (P<0.001) than values in all other groups, i.e. 35 553 (16 860–74 968) ng.ml–1. CONCLUSIONS: Our results suggest an antiestrogenic potential of propylparaben tested in vivo in juvenile zebrafish (Danio rerio). The estrogenic effect of 17B-estradiol was confirmed.

Abstract (in Czech)

OBJECTIVES: Xenoestrogenic potential of propylparaben (PP), one of the most commonly used preservatives in drugs, cosmetics and food, was investigated in vivo using zebrafish (Danio rerio). METHODS: Juvenile zebrafish (20 days post hatching) were exposed to three different concentrations of propylparaben (PP) dissolved in ethanol and added into the water. After 20 days of exposure the fish were euthanized and vitellogenin concentrations in their whole body homogenates were measured by the method of direct sandwich ELISA. Simultaneously, vitellogenin concentrations in either fish from the control group (exposed to solvent without the substance tested) and in fish from the positive control group (exposed to natural estrogen 17B-estradiol) were measured. RESULTS: Vitellogenin concentration in whole body homogenates of control fish was 400 (396–540) ng.ml–1 (geometric mean (95% CI)). Zebrafish exposure to propylparaben at the concentrations of 0.1; 0.4 and 0.9 mg.l–1 elicited statistically significant decline (P<0.001) of vitellogenin production, i.e. geometric means of vitellogenin concentrations in whole body homogenates were 240 (186–311); 218 (175–270) and 270 (234–311) ng.ml–1, respectively. Conversely, the geometric mean of vitellogenin concentration in whole body homogenates of zebrafish exposed to 100 ng.l–1 of 17B-estradiol (positive control) was significantly higher (P<0.001) than values in all other groups, i.e. 35 553 (16 860–74 968) ng.ml–1. CONCLUSIONS: Our results suggest an antiestrogenic potential of propylparaben tested in vivo in juvenile zebrafish (Danio rerio). The estrogenic effect of 17B-estradiol was confirmed.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)